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The Culture of Limbal Epithelial Cells

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Corneal Regenerative Medicine

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1014))

Abstract

The transplantation of cultured limbal epithelial cells (LEC) has since its first application in 1997 emerged as a promising technique for treating limbal stem cell deficiency. The culture methods hitherto used vary with respect to preparation of the harvested tissue, choice of culture medium, culture time, culture substrates, and supplementary techniques. In this chapter, we describe a procedure for establishing human LEC cultures using a feeder-free explant culture technique with human amniotic membrane (AM) as the culture substrate.

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Acknowledgments

The authors thank Astrid Østerud at the Department of Ophthalmology, Oslo University Hospital, Norway; Ingrid Riphagen at the Unit for Applied Clinical Research, Norwegian University of Science and Technology, Norway; Øygunn Aass Utheim and Jon Roger Eidet at the Department of Medical Biochemistry, Oslo University Hospital, Norway; Borghild Roald at the Department of Pathology, Oslo University Hospital, Norway; Edward Messelt at the Institute of Oral Biology, Dental Faculty, University of Oslo, Norway for excellent help and support.

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Authors and Affiliations

  1. Department of Medical Biochemistry, Oslo University Hospital, Oslo, Norway

    Tor Paaske Utheim & Torstein Lyberg

  2. Schepens Eye Research Institute, Massachusetts Eye and Ear, Department of Ophthalmology, Harvard Medical School, Boston, MA, USA

    Tor Paaske Utheim

  3. Center for Eye Research, Department of Ophthalmology, Ulleval University Hospital, University of Oslo, Oslo, Norway

    Sten Ræder

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  1. Tor Paaske Utheim
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  1. School of Pharmacy, University of Reading, Shinfield Road, Reading, RG6 6UB, United Kingdom

    Bernice Wright

  2. School of Pharmacy, University of Reading, Shinfield Road, Reading, RG6 6UB, United Kingdom

    Che J Connon

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Utheim, T.P., Lyberg, T., Ræder, S. (2013). The Culture of Limbal Epithelial Cells. In: Wright, B., Connon, C. (eds) Corneal Regenerative Medicine. Methods in Molecular Biology, vol 1014. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-432-6_7

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  • DOI: https://doi.org/10.1007/978-1-62703-432-6_7

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