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Microsatellites pp 67–87Cite as

Microsatellite DNA Capture from Enriched Libraries

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1006))

Abstract

Microsatellites are DNA sequences of tandem repeats of one to six nucleotides, which are highly polymorphic, and thus the molecular markers of choice in many kinship, population genetic, and conservation studies. There have been significant technical improvements since the early methods for microsatellite isolation were developed, and today the most common procedures take advantage of the hybrid capture methods of enriched-targeted microsatellite DNA. Furthermore, recent advents in sequencing technologies (i.e., next-generation sequencing, NGS) have fostered the mining of microsatellite markers in non-model organisms, affording a cost-effective way of obtaining a large amount of sequence data potentially useful for loci characterization. The rapid improvements of NGS platforms together with the increase in available microsatellite information open new avenues to the understanding of the evolutionary forces that shape genetic structuring in wild populations. Here, we provide detailed methodological procedures for microsatellite isolation based on the screening of GT microsatellite-enriched libraries, either by cloning and Sanger sequencing of positive clones or by direct NGS. Guides for designing new species-specific primers and basic genotyping are also given.

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Acknowledgments

EGG is sponsored by a postdoctoral fellowship of the Ministerio de Educación Cultura y Deporte (MECD). The research of R.Z. is funded by the Ministerio de Economía y Competitividad (CGL2010-18216).

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Authors and Affiliations

  1. Departamento de Biodiversidad y Biología Evolutiva, Museo Nacional de Ciencias Naturales, MNCN-CSIC, Madrid, Spain

    Elena G. Gonzalez & Rafael Zardoya

  2. CCMAR, Universidade do Algarve, Faro, Portugal

    Elena G. Gonzalez

Authors
  1. Elena G. Gonzalez
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  2. Rafael Zardoya
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Editor information

Editors and Affiliations

  1. , Department of Plant, Soil,, Southern Illinois University, Carbondale, Lincoln Drive 1205, Carbondale, 62901, Illinois, USA

    Stella K. Kantartzi

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Gonzalez, E.G., Zardoya, R. (2013). Microsatellite DNA Capture from Enriched Libraries. In: Kantartzi, S. (eds) Microsatellites. Methods in Molecular Biology, vol 1006. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-389-3_5

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  • DOI: https://doi.org/10.1007/978-1-62703-389-3_5

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-388-6

  • Online ISBN: 978-1-62703-389-3

  • eBook Packages: Springer Protocols

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