Abstract
Myocardial infarction (MI) is death and necrosis of myocardial tissue secondary to ischemia. MI is associated with adverse cardiac remodeling, progressive heart chamber dilation, ventricular wall thinning, and loss of cardiac function. Myocardial necrosis can be experimentally induced in rodents to simulate human MI by surgical occlusion of coronary arteries. When induced in knockout or transgenic mice, this model is useful for the identification of molecular modulators of cell death, cardiac remodeling, and preclinical therapeutic potential. Herein we outline in tandem, methods for microsurgical ligation of the left anterior descending artery followed by quantitation of myocardial necrosis. Necrosis is quantified after staining the heart with triphenyltetrazolium chloride.
Xin-Yi Yeap and Shirley Dehn have equally contributed to this work.
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Acknowledgements
Thank-you to members of the Feinberg Cardiovascular Research Institute, including Sol Misener, Prasanna Krishnamurthy, and Alex Mackie. Thank-you also to Jackie Schriewer. Funding from NIH 4R00HL097021-03 grant from the NHLBI (to E.T.).
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Yeap, XY., Dehn, S., Adelman, J., Lipsitz, J., Thorp, E.B. (2013). Quantitation of Acute Necrosis After Experimental Myocardial Infarction. In: McCall, K., Klein, C. (eds) Necrosis. Methods in Molecular Biology, vol 1004. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-383-1_9
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DOI: https://doi.org/10.1007/978-1-62703-383-1_9
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