A Robust, Rapid, and Simple Method of Producing Olfactory Receptors Using Commercial E. coli Cell-Free Systems

  • Karolina Corin
  • Brian Cook
  • Shuguang Zhang
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1003)

Abstract

The first bottleneck in olfactory receptor (OR) studies is producing sufficient quantities of soluble, ­functional, and stable receptors. Commercial cell-free in vitro translation systems can be used to produce milligrams of soluble and functional receptors within several hours directly from plasmid DNA. The receptors can be purified using immunoaffinity chromatography and gel filtration, and can be analyzed using gel electrophoresis and with other standard techniques.

Key words

In vitro translation Olfactory receptors Odorant ligand-binding Microscale thermophoresis 

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Karolina Corin
    • 1
  • Brian Cook
    • 1
  • Shuguang Zhang
    • 1
  1. 1.Center for Bits and Atoms, Massachusetts Institute of TechnologyCambridgeUSA

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