Pharmacology of Mammalian Olfactory Receptors

  • Richard S. Smith
  • Zita Peterlin
  • Ricardo C. Araneda
Part of the Methods in Molecular Biology book series (MIMB, volume 1003)


Mammalian species have evolved a large and diverse number of odorant receptors (ORs). These proteins comprise the largest family of G-protein-coupled receptors (GPCRs) known, amounting to ∼1,000 ­different receptors in the rodent. From the perspective of olfactory coding, the availability of such a vast number of chemosensory receptors poses several fascinating questions; in addition, such a large repertoire provides an attractive biological model to study ligand–receptor interactions. The limited functional expression of these receptors in heterologous systems, however, has greatly hampered attempts to deorphanize them. We have employed a successful approach that combines electrophysiological and imaging techniques to analyze the response profiles of single sensory neurons. Our approach has enabled us to characterize the “odor space” of a population of native aldehyde receptors and the molecular range of a genetically engineered receptor, OR-I7.

Key words

Olfactory receptors Aldehydes OR-I7 Sensory neurons Ca-imaging 



The authors would like to thank Xinmin Zhang for his helpful input in developing and optimizing these procedures.


  1. 1.
    Malnic B, Hirono J, Sato T, Buck LB (1999) Combinatorial receptor codes for odors. Cell 96:713–723PubMedCrossRefGoogle Scholar
  2. 2.
    Mombaerts P (2004) Genes and ligands for odorant, vomeronasal and taste receptors. Nat Rev Neurosci 5:263–278PubMedCrossRefGoogle Scholar
  3. 3.
    Reisert J, Restrepo D (2009) Molecular tuning of odorant receptors and its implication for odor signal processing. Chem Senses 34:535–545PubMedCrossRefGoogle Scholar
  4. 4.
    Touhara K (2007) Deorphanizing vertebrate olfactory receptors: recent advances in odorant-response assays. Neurochem Int 51: 132–139PubMedCrossRefGoogle Scholar
  5. 5.
    Matsunami H, Mainland JD, Dey S (2009) Trafficking of mammalian chemosensory receptors by receptor-transporting proteins. Ann N Y Acad Sci 1170:153–156PubMedCrossRefGoogle Scholar
  6. 6.
    Araneda RC, Peterlin Z, Zhang X, Chesler A, Firestein S (2004) A pharmacological profile of the aldehyde receptor repertoire in rat olfactory epithelium. J Physiol 555: 743–756PubMedCrossRefGoogle Scholar
  7. 7.
    Kajiya K, Inaki K, Tanaka M, Haga T, Kataoka H, Touhara K (2001) Molecular bases of odor discrimination: reconstitution of olfactory receptors that recognize overlapping sets of odorants. J Neurosci 21:6018–6025PubMedGoogle Scholar
  8. 8.
    Kaluza JF, Breer H (2000) Responsiveness of olfactory neurons to distinct aliphatic aldehydes. J Exp Biol 203(Pt 5):927–933PubMedGoogle Scholar
  9. 9.
    Araneda RC, Kini AD, Firestein S (2000) The molecular receptive range of an odorant receptor. Nat Neurosci 3:1248–1255PubMedCrossRefGoogle Scholar
  10. 10.
    Yuste R, Lanni F, Konnerth A (2000) Imaging neurons: a laboratory manual. Cold Spring Harbor Laboratory Press, New YorkGoogle Scholar
  11. 11.
    Buck L, Axel R (1991) A novel multigene ­family may encode odorant receptors: a molecular basis for odor recognition. Cell 65:175–187PubMedCrossRefGoogle Scholar
  12. 12.
    McClintock TS, Sammeta N (2003) Trafficking prerogatives of olfactory receptors. Neuroreport 14:1547–1552PubMedCrossRefGoogle Scholar
  13. 13.
    Saito H, Kubota M, Roberts RW, Chi Q, Matsunami H (2004) RTP family members induce functional expression of mammalian odorant receptors. Cell 119:679–691PubMedCrossRefGoogle Scholar
  14. 14.
    Peterlin Z, Li Y, Sun G, Shah R, Firestein S, Ryan K (2008) The importance of odorant conformation to the binding and activation of a representative olfactory receptor. Chem Biol 15:1317–1327PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Richard S. Smith
    • 1
  • Zita Peterlin
    • 2
  • Ricardo C. Araneda
    • 1
  1. 1.Department of BiologyUniversity of MarylandCollege ParkUSA
  2. 2.Department of BiologyColumbia UniversityNew YorkUSA

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