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Microelectrode Biosensors pp 153–177Cite as

Monitoring Extracellular Glutamate in the Brain by Microdialysis and Microsensors

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Part of the book series: Neuromethods ((NM,volume 80))

Abstract

Recording basal levels of extracellular glutamate by microdialysis in the brain of freely moving animals is of limited value as the result does not fulfill the criteria of synaptic origin (TTX or calcium dependency). In this chapter we discuss whether the recently developed microsensor technique provides an alternative for sampling glutamate by microdialysis. Although glutamate microsensors show advantages in dimensional and temporal resolution, the properties of the detected glutamate do not differ basically from glutamate in microdialysate. The results suggest that glutamate detected by both microdialysis and microsensoring methods is closely regulated by non-neuronal cells. However, there is some evidence that evoked glutamate release recorded by microsensors (e.g., by elevated potassium or behavioral simulation), in the time frame of seconds, might reflect neuronal release.

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Authors and Affiliations

  1. Department of Pharmacy, University of Groningen, Groningen, The Netherlands

    Si Qin, Miranda (van der Zeyden) Evering, Ngabi Wahono, Thomas I. F. H. Cremers & Ben H. C. Westerink

  2. Brains On-Line BV, Groningen, The Netherlands

    Si Qin, Miranda (van der Zeyden) Evering, Ngabi Wahono, Thomas I. F. H. Cremers & Ben H. C. Westerink

Authors
  1. Si Qin
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  2. Miranda (van der Zeyden) Evering
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  3. Ngabi Wahono
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  4. Thomas I. F. H. Cremers
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  5. Ben H. C. Westerink
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Editors and Affiliations

  1. Faculté de médecine, CRNL, Université Claude Bernard Lyon I, Avenue Rockefeller 8, Lyon, 69373, France

    Stéphane Marinesco

  2. School of Life Sciences, University of Warwick, Gibbet Hill Road, Coventry, CV4 7AL, United Kingdom

    Nicholas Dale

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Qin, S., Evering, M.(., Wahono, N., Cremers, T.I.F.H., Westerink, B.H.C. (2013). Monitoring Extracellular Glutamate in the Brain by Microdialysis and Microsensors. In: Marinesco, S., Dale, N. (eds) Microelectrode Biosensors. Neuromethods, vol 80. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-370-1_7

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  • DOI: https://doi.org/10.1007/978-1-62703-370-1_7

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-369-5

  • Online ISBN: 978-1-62703-370-1

  • eBook Packages: Springer Protocols

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