Organ Regeneration pp 81-97 | Cite as
Xeno-Free Adaptation and Culture of Human Pluripotent Stem Cells
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Abstract
Human pluripotent stem cells (hPSCs), including embryonic stem (ES) and induced pluripotent stem (iPS) cells, have been historically cultured in media containing xenogeneic animal components. As hPSC-derived cells and tissues are being developed for human therapies, the application of culture systems to reduce potential immunoreactivity and improve reproducibility becomes increasingly vital. Methods for directly adapting hPSCs to a commercially available culture system free of nonhuman proteins (xeno-free) are described in this chapter.
Key words
Human embryonic stem cell Induced pluripotent stem cell Human pluripotent stem cell Xeno-freeNotes
Acknowledgments
The author extends many thanks to Marian Piekarczyk for her significant contributions to this work and critical review of the chapter. Thanks to David Piper for his encouragement and critical review of the chapter. Thanks to Justin Wetter and Jun Wang for their assistance with hPSC culture and flow cytometry. Lastly, thanks to my family for their love and support.
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