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A “Living Bioreactor” for the Production of Tissue-Engineered Small Intestine

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Book cover Organ Regeneration

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1001))

Abstract

Here, we describe the use of a mouse model as a living bioreactor for the generation of tissue-engineered small intestine. Small intestine is harvested from donor mice with subsequent isolation of organoid units (a cluster of mesenchymal and epithelial cells). Some of these organoid units contain pluripotent stem cells with a preserved relationship with the mesenchymal stem cell niche. A preparation of organoid units is seeded onto a biodegradable scaffold and implanted intraperitoneally within the omentum of the host animal. The cells are nourished initially via imbibition until neovascularization occurs. This technique allows the growth of fully differentiated epithelium (composed of Paneth cells, goblet cells, enterocytes and enteroendocrine cells), muscle, nerve, and blood vessels of donor origin. Variations of this technique have been used to generate tissue-engineered stomach, large intestine, and esophagus. The variations include harvest technique, length of digestion, and harvest times.

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Acknowledgments

This work was supported by grants from the California Institute for Regenerative Medicine (#RN2-00946-1) (TG2-01168) and the Saban Research Institute

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Authors and Affiliations

  1. Division of Pediatric Surgery, Saban Research Institute, Children’s Hospital Los Angeles, Keck School of Medicine of the University of Southern California, Los Angeles, CA, USA

    Daniel E. Levin, Frederic G. Sala, Erik R. Barthel, Allison L. Speer, Xiaogang Hou, Yasuhiro Torashima & Tracy C. Grikscheit

Authors
  1. Daniel E. Levin
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  2. Frederic G. Sala
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  3. Erik R. Barthel
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  4. Allison L. Speer
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  5. Xiaogang Hou
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  6. Yasuhiro Torashima
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  7. Tracy C. Grikscheit
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Editor information

Editors and Affiliations

  1. Tengion, Inc, Westpoint Blvd. 3929, Winston-Salem, 27103, North Carolina, USA

    Joydeep Basu

  2. Zen Bio, Inc., E Hwy 54 3200, Research Triangle Park, 27709, North Carolina, USA

    John W. Ludlow

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Levin, D.E. et al. (2013). A “Living Bioreactor” for the Production of Tissue-Engineered Small Intestine. In: Basu, J., Ludlow, J. (eds) Organ Regeneration. Methods in Molecular Biology, vol 1001. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-363-3_25

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  • DOI: https://doi.org/10.1007/978-1-62703-363-3_25

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-362-6

  • Online ISBN: 978-1-62703-363-3

  • eBook Packages: Springer Protocols

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