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Human Liver Bioengineering Using a Whole Liver Decellularized Bioscaffold

Part of the Methods in Molecular Biology book series (MIMB,volume 1001)

Abstract

As a result of significant progress made in the last years in developing methods of whole organ decellularization techniques, organ bioengineering may now look more feasible than ever before. In this chapter, we describe in detail the necessary steps in human liver bioengineering. These include ferret liver decellularization by detergent perfusion, human liver progenitor and endothelial cell isolation, and finally, liver bioscaffold recellularization in a perfusion bioreactor.

Key words

  • Human liver progenitors
  • Decellularization
  • Scaffold
  • Bioengineering
  • Endothelial cells
  • Perfusion bioreactor
  • Whole organ
  • Recellularization

*Pedro M. Baptista and Dipen Vyas have contributed equally to this work.

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  • DOI: 10.1007/978-1-62703-363-3_24
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References

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  4. Baptista PM, Siddiqui MM, Lozier G et al (2011) The use of whole organ decellularization for the generation of a vascularized liver organoid. Hepatology 53(2):604–617

    PubMed  CrossRef  CAS  Google Scholar 

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Baptista, P.M., Vyas, D., Moran, E., Wang, Z., Soker, S. (2013). Human Liver Bioengineering Using a Whole Liver Decellularized Bioscaffold. In: Basu, J., Ludlow, J. (eds) Organ Regeneration. Methods in Molecular Biology, vol 1001. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-363-3_24

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  • DOI: https://doi.org/10.1007/978-1-62703-363-3_24

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-362-6

  • Online ISBN: 978-1-62703-363-3

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