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Tissue Preparation for MALDI-MS Imaging of Protein and Peptides

  • Simona Colantonio
  • Roberta M. Smith
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1002)

Abstract

Matrix-assisted laser desorption/ionization (MALDI) imaging is rapidly gaining importance in the ­biomarkers field because it is able to detect several analytes at the same time and to assign to each one of them not only an m/z value but also spatial distribution. Here we present the detailed description of sample preparation for protein and peptide MALDI imaging assays. This chapter describes the microtomy performed in a cryostat to produce tissue slides mounted onto glass slides suitable for MALDI. Sample preparation will include matrix coating procedure with a sensor-controlled aerosol. Finally, we will show some examples of how data can be visualized to suit the purposes of the research.

Key words

Mass spectrometry MALDI Imaging Mouse Protein Peptide 

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Simona Colantonio
    • 1
  • Roberta M. Smith
    • 2
  1. 1.Protein Chemistry Laboratory, Advance Technology Program, SAIC-Frederick, Frederick National Laboratory for Cancer ResearchFrederickUSA
  2. 2.Pathology/Histology Laboratory, SAIC-Frederick Inc., Frederick National Laboratory for Cancer ResearchFrederickUSA

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