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Tissue Sample Preparation for Biomarker Discovery

  • Yoshiyuki Suehara
  • Daisuke Kubota
  • Tsuyoshi Saito
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1002)

Abstract

Global protein expression studies, an approach known as “proteomics,” can offer important clues for understanding tumor biology that cannot be obtained by other approaches. Proteomic studies have provided protein expression profiles of tumors that can be used to develop novel diagnostic and therapeutic biomarkers. In this chapter, we describe the strategy and design of proteomic studies, as well as the protocols for tissue sample collection and preparation for biomarker discovery, especially tumor biomarkers, followed by a few examples of our recent proteomic studies.

Key words

Proteomics 2D-DIGE Tissue samples preparation Soft tissue sarcomas 

Notes

Acknowledgments

This work was supported by a grant from the Japan Society for the Promotion of Science (JSPS), a science Grant-in-Aid for Young Scientists B, No-22791405. The authors appreciate critical comments and support from Dr. Makoto Endo and Dr. Eisuke Kobayashi.

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Yoshiyuki Suehara
    • 1
  • Daisuke Kubota
    • 1
  • Tsuyoshi Saito
    • 2
  1. 1.Department of Orthopedic SurgeryJuntendo University School of MedicineTokyoJapan
  2. 2.Department of Human PathologyJuntendo University School of MedicineTokyoJapan

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