Global protein expression studies, an approach known as “proteomics,” can offer important clues for understanding tumor biology that cannot be obtained by other approaches. Proteomic studies have provided protein expression profiles of tumors that can be used to develop novel diagnostic and therapeutic biomarkers. In this chapter, we describe the strategy and design of proteomic studies, as well as the protocols for tissue sample collection and preparation for biomarker discovery, especially tumor biomarkers, followed by a few examples of our recent proteomic studies.
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This work was supported by a grant from the Japan Society for the Promotion of Science (JSPS), a science Grant-in-Aid for Young Scientists B, No-22791405. The authors appreciate critical comments and support from Dr. Makoto Endo and Dr. Eisuke Kobayashi.
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