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Rational-Based Protein Engineering: Tips and Tools

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Protein Nanotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 996))

Abstract

The rational engineering of proteins is driven by contemporary needs for new and altered biomolecular forms. Utilizing manipulative procedures of molecular biology, it is relatively straightforward to alter protein structure and function to create mutated or fused sequences. We here give an overview of procedures and strategies for site-directed mutagenesis, construction of fusion proteins, and insertion of tags. The design of new protein constructs as well as their over-expression as recombinant products is considered. We also summarize approaches for the engineering of protein complexes by co-expression, a valuable route to generate bioactive multicomponent systems.

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Authors and Affiliations

  1. Structural and Computational Biology Division, Victor Chang Cardiac Research Institute, Sydney, Australia

    Meghna Sobti

  2. Department of Chemistry and Biomolecular Sciences, Macquarie University, Sydney, Australia

    Bridget C. Mabbutt

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  1. Meghna Sobti
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  2. Bridget C. Mabbutt
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Editors and Affiliations

  1. University of Canterbury, Kirkwood Avenue 20, Christchurch, 8041, New Zealand

    Juliet A. Gerrard

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Sobti, M., Mabbutt, B.C. (2013). Rational-Based Protein Engineering: Tips and Tools. In: Gerrard, J. (eds) Protein Nanotechnology. Methods in Molecular Biology, vol 996. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-354-1_14

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  • DOI: https://doi.org/10.1007/978-1-62703-354-1_14

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