Advertisement

Feeder-Free Substrates for Pluripotent Stem Cell Culture

  • Alexandria Sams
  • Mark J. Powers
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 997)

Abstract

A significant barrier to the therapeutic application of pluripotent stem cells (PSCs) is the risk associated with the presence of undefined, animal-derived elements that are routinely used to culture these cells. Originally, PSCs were derived on mouse feeder cells in media containing fetal calf serum. Such conditions could expose potential patients to animal pathogens or lead to immune rejection. Substantial efforts have been made to remove these components and successfully maintain these cells in a completely defined, xeno-free environment. In this chapter, we examine substrates consisting of animal-derived proteins, purified human proteins, recombinant human proteins, and synthetic polymers and their ability to maintain the undifferentiated growth of various pluripotent stem cell lines in a variety of supplemented media.

Key words

Human, Pluripotent stem cells, Culture, Media, Matrix 

References

  1. 1.
    Thomson JA, Itskovitz-Eldor J, Shapiro SS, Waknitz MA, Swiergiel JJ, Marshall VS, Jones JM (1998) Science 282:1145–1147PubMedCrossRefGoogle Scholar
  2. 2.
    Gepstein L (2002) Circ Res 91:866–876PubMedCrossRefGoogle Scholar
  3. 3.
    Takahashi K, Yamanaka S (2006) Cell 126:663–676PubMedCrossRefGoogle Scholar
  4. 4.
    Richards M, Tan S, Fong CY, Biswas A, Chan WK, Bongso A (2003) Stem Cells 21:546–556PubMedCrossRefGoogle Scholar
  5. 5.
    Meng G, Liu S, Rancourt DE (2012) Stem Cells Dev 21:2036–2048Google Scholar
  6. 6.
    Tavakoli T, Xu X, Derby E, Serebryakova Y, Reid Y, Rao MS, Mattson MP, Ma W (2009) BMC Cell Biol 10:44PubMedCrossRefGoogle Scholar
  7. 7.
    Chang KH, Nelson AM, Fields PA, Hesson JL, Ulyanova T, Cao H, Nakamoto B, Ware CB, Papayannopoulou T (2008) Exp Cell Res 314:2930–2940PubMedCrossRefGoogle Scholar
  8. 8.
    Allegrucci C, Young LE (2007) Hum Reprod Update 13:103–120PubMedCrossRefGoogle Scholar
  9. 9.
    Xu C, Inokuma MS, Denham J, Golds K, Kundu P, Gold JD, Carpenter MK (2001) Nat Biotechnol 19:971–974PubMedCrossRefGoogle Scholar
  10. 10.
    Hughes CS, Postovit LM, Lajoie GA (2010) Proteomics 10:1886–1890PubMedCrossRefGoogle Scholar
  11. 11.
    Stewart MH, Bendall SC, Bhatia M (2008) J Mol Med 86:875–886PubMedCrossRefGoogle Scholar
  12. 12.
    Xu C, Rosler E, Jiang J, Lebkowski JS, Gold JD, O’Sullivan C, Delavan-Boorsma K, Mok M, Bronstein A, Carpenter MK (2005) Stem Cells 23:315–323PubMedCrossRefGoogle Scholar
  13. 13.
    Xu RH, Peck RM, Li DS, Feng X, Ludwig T, Thomson JA (2005) Nat Methods 2:185–190PubMedCrossRefGoogle Scholar
  14. 14.
    Wang L, Li L, Menendez P, Cerdan C, Bhatia M (2005) Blood 105:4598–4603PubMedCrossRefGoogle Scholar
  15. 15.
    Klagsbrun M, Baird A (1991) Cell 67:229–231PubMedCrossRefGoogle Scholar
  16. 16.
    Dvorák P, Hampl A, Jirmanová L, Pacholíková J, Kusakabe M (1998) J Cell Sci 111:2945–2952PubMedGoogle Scholar
  17. 17.
    Yu J, Vodyanik MA, Smuga-Otto K, Antosiewicz-Bourget J, Frane JL, Tian S, Nie J, Jonsdottir GA, Ruotti V, Stewart R, Slukvin II, Thomson JA (2007) Science 318:1917–1920PubMedCrossRefGoogle Scholar
  18. 18.
    Rowland TJ, Miller LM, Blaschke AJ, Doss EL, Bonham AJ, Hikita ST, Johnson LV, Clegg DO (2010) Stem Cells Dev 19:1231–1240PubMedCrossRefGoogle Scholar
  19. 19.
    Jones MB, Chu C, Pendleton JC, Betenbaugh MJ, Shiloach J, Baljinnyam B, Rubin JS, Shamblott MJ (2010) Stem Cells Dev 19:1923–1935PubMedCrossRefGoogle Scholar
  20. 20.
    Furue MK, Na J, Jackson JP, Okamoto T, Jones M, Baker D, Hata R, Moore HD, Sato JD, Andrews PW (2008) Proc Natl Acad Sci U S A 105:13409–13414PubMedCrossRefGoogle Scholar
  21. 21.
    Stojkovic P, Lako M, Przyborski S, Stewart R, Armstrong L, Evans J, Zhang X, Stojkovic M (2005) Stem Cells 23:895–902PubMedCrossRefGoogle Scholar
  22. 22.
    Dziadek M, Timpl R (1985) Dev Biol 111:372–382PubMedCrossRefGoogle Scholar
  23. 23.
    Beattie GM, Lopez AD, Bucay N, Hinton A, Firpo MT, King CC, Hayek A (2005) Stem Cells 23:489–495PubMedCrossRefGoogle Scholar
  24. 24.
    Li Y, Powell S, Brunette E, Lebkowski J, Mandalam R (2005) Biotechnol Bioeng 91:688–698PubMedCrossRefGoogle Scholar
  25. 25.
    Amit M, Shariki C, Margulets V, Itskovitz-Eldor J (2004) Biol Reprod 70:837–845PubMedCrossRefGoogle Scholar
  26. 26.
    Noaksson K, Zoric N, Zeng X, Rao MS, Hyllner J, Semb H, Kubista M, Sartipy P (2005) Stem Cells 23:1460–1467PubMedCrossRefGoogle Scholar
  27. 27.
    Tsutsui H, Valamehr B, Hindoyan A, Qiao R, Ding X, Guo S, Witte ON, Liu X, Ho CM, Wu H (2011) Nat Commun 2:167PubMedCrossRefGoogle Scholar
  28. 28.
    Preissner KT, Reuning U (2011) Semin Thromb Hemost 37:408–424PubMedCrossRefGoogle Scholar
  29. 29.
    Hayman EG, Pierschbacher MD, Suzuki S, Ruoslahti E (1985) Exp Cell Res 160:245–258PubMedCrossRefGoogle Scholar
  30. 30.
    Braam SR, Zeinstra L, Litjens S, ward-van Oostwaard D, van den Brink S, van Laake L, Lebrin F, Kats P, Hochstenbach R, Passier R, Sonnenberg A, Mummery CL (2008) Stem Cells 26:2257–2265PubMedCrossRefGoogle Scholar
  31. 31.
    Chen G, Gulbranson DR, Hou Z, Bolin JM, Ruotti V, Probasco MD, Smuga-Otto K, Howden SE, Diol NR, Propson NE, Wagner R, Lee GO, Antosiewicz-Bourget J, Teng JM, Thomson JA (2011) Nat Methods 8:424–429PubMedCrossRefGoogle Scholar
  32. 32.
    Swistowski A, Peng J, Han Y, Swistowska AM, Rao MS, Zeng X (2009) PLoS One 4:e6233PubMedCrossRefGoogle Scholar
  33. 33.
    Miyazaki T, Futaki S, Hasegawa K, Kawasaki M, Sanzen N, Hayashi M, Kawase E, Sekiguchi K, Nakatsuji N, Suemori H (2008) Biochem Biophys Res Commun 375:27–32PubMedCrossRefGoogle Scholar
  34. 34.
    Rodin S, Domogatskaya A, Ström S, Hansson EM, Chien KR, Inzunza J, Hovatta O, Tryggvason K (2010) Nat Biotechnol 28:611–615PubMedCrossRefGoogle Scholar
  35. 35.
    Eastham AM, Spencer H, Soncin F, Ritson S, Merry CL, Stern PL, Ward CM (2007) Cancer Res 67:11254–11262PubMedCrossRefGoogle Scholar
  36. 36.
    Soncin F, Ward CM (2011) Genes 2:229–259CrossRefGoogle Scholar
  37. 37.
    Nagaoka M, Koshimizu U, Yuasa S, Hattori F, Chen H, Tanaka T, Okabe M, Fukuda K, Akaike T (2006) PLoS One 1:e15PubMedCrossRefGoogle Scholar
  38. 38.
    Nagaoka M, Si-Tayeb K, Akaike T, Duncan SA (2010) BMC Dev Biol 10:60PubMedCrossRefGoogle Scholar
  39. 39.
    Bongso A, Fong CY, Gauthaman K (2008) J Cell Biochem 105:1352–1360PubMedCrossRefGoogle Scholar
  40. 40.
    Banu N, Rosenzweig M, Kim H, Bagley J, Pykett M (2001) Cytokine 13:349–358PubMedCrossRefGoogle Scholar
  41. 41.
    Curran JM, Chen R, Hunt JA (2006) Biomaterials 27:4783–4793PubMedCrossRefGoogle Scholar
  42. 42.
    Brafman DA, Chang CW, Fernandez A, Willert K, Varghese S, Chien S (2010) Biomaterials 31:9135–9144PubMedCrossRefGoogle Scholar
  43. 43.
    Villa-Diaz LG, Nandivada H, Ding J, Nogueira-de-Souza NC, Krebsbach PH, O’Shea KS, Lahann J, Smith GD (2010) Nat Biotechnol 28:581–583PubMedCrossRefGoogle Scholar
  44. 44.
    Melkoumian Z, Weber JL, Weber DM, Fadeev AG, Zhou Y, Dolley-Sonneville P, Yang J, Qiu L, Priest CA, Shogbon C, Martin AW, Nelson J, West P, Beltzer JP, Pal S, Brandenberger R (2010) Nat Biotechnol 28:606–610PubMedCrossRefGoogle Scholar
  45. 45.
    Derda R, Musah S, Orner BP, Klim JR, Li L, Kiessling LL (2010) J Am Chem Soc 132:1289–1295PubMedCrossRefGoogle Scholar
  46. 46.
    Klim JR, Li L, Wrighton PJ, Piekarczyk MS, Kiessling LL (2010) Nat Methods 7:989–994PubMedCrossRefGoogle Scholar
  47. 47.
    Saha K, Mei Y, Reisterer CM, Pyzocha NK, Yang J, Muffat J, Davies MC, Alexander MR, Langer R, Anderson DG, Jaenisch R (2011) Proc Natl Acad Sci U S A 108:18714–18719PubMedCrossRefGoogle Scholar
  48. 48.
    Meng Y, Eshghi S, Li YJ, Schmidt R, Schaffer DV, Healy KE (2010) FASEB J 24:1056–1065PubMedCrossRefGoogle Scholar
  49. 49.
    Rosler ES, Fisk GJ, Ares X, Irving J, Miura T, Rao MS, Carpenter MK (2004) Dev Dyn 229:259–274PubMedCrossRefGoogle Scholar
  50. 50.
    Peerani R, Rao BM, Bauwens C, Yin T, Wood GA, Nagy A, Kumacheva E, Zandstra PW (2007) EMBO J 26:4744–4755PubMedCrossRefGoogle Scholar
  51. 51.
    Vallier L, Alexander M, Pedersen RA (2005) J Cell Sci 118:4495–4509PubMedCrossRefGoogle Scholar
  52. 52.
    Xiao L, Yuan X, Sharkis SJ (2006) Stem Cells 24:1476–1486PubMedCrossRefGoogle Scholar
  53. 53.
    Yao S, Chen S, Clark J, Hao E, Beattie GM, Hayek A, Ding S (2006) Proc Natl Acad Sci U S A 103:6907–6912PubMedCrossRefGoogle Scholar
  54. 54.
    Levenstein ME, Ludwig TE, Xu RH, Llanas RA, VanDenHeuvel-Kramer K, Manning D, Thomson JA (2006) Stem Cells 24:568–574PubMedCrossRefGoogle Scholar
  55. 55.
    Bendall SC, Stewart MH, Menendez P, George D, Vijayaragavan K, Werbowetski-Ogilvie T, Ramos-Mejia V, Rouleau A, Yang J, Bosse M, Lajoie G, Bhatia M (2007) Nature 448:1015–1021PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Alexandria Sams
    • 1
  • Mark J. Powers
    • 1
  1. 1.Primary and Stem Cell SystemsLife TechnologiesFrederickUSA

Personalised recommendations