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Generation of Human-Induced Pluripotent Stem Cells (hiPSCs) Using Episomal Vectors on Defined Essential 8™ Medium Conditions

  • Andrew Fontes
  • Chad C. MacArthur
  • Pauline T. Lieu
  • Mohan C. Vemuri
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 997)

Abstract

Human-induced pluripotent stem cells (iPSCs) are an important potential source of cells for regenerative medicine due to their inherent ability to differentiate into all cell types of the three germ layers. Generation of iPSCs with a non-integrating reprogramming method and in culture conditions that are completely absent of animal proteins will be ideal for such regenerative and cell therapy applications. Here we describe a method to generate non-integrating iPSCs using the Episomal iPSC Reprogramming Vectors.

Key words

iPSC Episomal vectors Essential 8 medium Pluripotency 

References

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Andrew Fontes
    • 1
  • Chad C. MacArthur
    • 1
  • Pauline T. Lieu
    • 1
  • Mohan C. Vemuri
    • 2
  1. 1.Primary and Stem Cell SystemsLife TechnologiesCarlsbadUSA
  2. 2.Primary and Stem Cell SystemsLife TechnologiesFrederickUSA

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