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Haemostasis pp 407-412 | Cite as

Testing for Apolipoprotein(a) Phenotype Using Isoelectric Focusing and Immunoblotting Technique

  • Claus Langer
  • Bertram Tambyrayah
  • Ulrike Nowak-Göttl
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 992)

Abstract

Increased levels of lipoprotein(a) are known as an independent risk factor for atherosclerosis, heart disease, and stroke in man. Even in children it could show that elevated levels of Lp(a) are an independent thromboembolic risk factor.

Levels of Lp(a) are influenced by several factors like nutrition, kidney or liver function, or acute-phase reaction. But the most important factors are genetically determined. About 45% of genetic variation depends on polymorphisms and mutations in the promotor region. About 50% are dependent on the size polymorphism of Lp(a). The number of Kringle 4 domains varies between 12 and over 40. The number of Kringle 4 repeats correlates negatively with the level of Lp(a) in plasma. The determination of apo(a) phenotype is able to estimate thromboembolic risk due to this risk factor.

Key words

Apolipoprotein(a) Phenotyping Isoelectric focusing Immunoblotting technique Thromboembolic risk factor 

References

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    Kambouh MI, Ferrel RE, Kottke BA (1991) Expressed hypervariable polymorphism of apolipoprotein (a). Am J Hum Genet 49:1063–1074Google Scholar

Copyright information

© Humana Press 2013

Authors and Affiliations

  • Claus Langer
    • 1
  • Bertram Tambyrayah
    • 2
  • Ulrike Nowak-Göttl
    • 3
  1. 1.Department of Laboratory MedicineUniversity HospitalMunsterGermany
  2. 2.Hemostasis and Thrombosis UnitUniversity Hospital Schleswig-HolsteinKielGermany
  3. 3.Thrombosis and Hemostasis Treatment CenterUniversity Hospital Schleswig-HolsteinKielGermany

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