Testing for Apolipoprotein(a) Phenotype Using Isoelectric Focusing and Immunoblotting Technique
Increased levels of lipoprotein(a) are known as an independent risk factor for atherosclerosis, heart disease, and stroke in man. Even in children it could show that elevated levels of Lp(a) are an independent thromboembolic risk factor.
Levels of Lp(a) are influenced by several factors like nutrition, kidney or liver function, or acute-phase reaction. But the most important factors are genetically determined. About 45% of genetic variation depends on polymorphisms and mutations in the promotor region. About 50% are dependent on the size polymorphism of Lp(a). The number of Kringle 4 domains varies between 12 and over 40. The number of Kringle 4 repeats correlates negatively with the level of Lp(a) in plasma. The determination of apo(a) phenotype is able to estimate thromboembolic risk due to this risk factor.
Key wordsApolipoprotein(a) Phenotyping Isoelectric focusing Immunoblotting technique Thromboembolic risk factor
- 1.Kostner GM (2002) Lipoprotein(a): metabolismus und beeinflussung des plasmaspiegels. J Kardiol 9:321–324Google Scholar
- 3.Marcovina SM, Hobbs HH, Albers JJ (1996) Relation between number of apolipoprotein (a) kringle 4 repeats and mobility of isoforms in agarose gel: basis for a standardized isoform nomenclature. Clin Chem 42:499–502Google Scholar
- 4.Kambouh MI, Ferrel RE, Kottke BA (1991) Expressed hypervariable polymorphism of apolipoprotein (a). Am J Hum Genet 49:1063–1074Google Scholar