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Haemostasis pp 349-351 | Cite as

Platelet Neutralization Test

  • Kottayam Radhakrishnan
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 992)

Abstract

The platelet neutralization test is used in the laboratorvy diagnosis of the lupus anticoagulant (Laffan and Manning, Dacie and Lewis practical haematology. Churchill Livingstone, Philadelphia, PA, pp 445–446, 2006). The lupus anticoagulant typically causes prolongation of phospholipid-dependent coagulation tests such as APTT, DRVVT, etc.

The phospholipid-dependent nature of the LA can be demonstrated by adding washed and “fractured” platelets as a source of phospholipid and repeating the tests. If an LA is present in the sample, the addition of platelets will correct the prolonged clotting times. This appears to be due to the ability of the platelets to absorb the LA and negate its effect on the clotting time (Br J Haematol 109:704–715, 2001).

Key words

Platelet neutralization Phospholipid Lupus anticoagulant Clotting time Activated ­partial thromboplastin time Dilute Russell’s viper venom time Centrifuge 

References

  1. 1.
    Laffan M, Manning R (2006) Investigation of a thrombotic tendency. In: Lewis SM, Bain BJ, Bates I (eds) Dacie and Lewis practical haematology. Churchill Livingstone, Philadelphia, PA, pp 445–446Google Scholar
  2. 2.
    Greaves M, Cohen H, Machin SJ, Mackie I (2000) Guidelines on the investigation and management of the antiphospholipid syndrome. Br J Haematol. 109:704–715Google Scholar
  3. 3.
    Pengo V, Tripodi A, Reber G, Rand JH, Ortel L, Galli M, De Groot PG (2009) Update of the guidelines for lupus anticoagulant detection. J Thromb Haemost 7:1737–1740PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press 2013

Authors and Affiliations

  • Kottayam Radhakrishnan
    • 1
  1. 1.Department of Clinical HaematologyRoyal Children’s HospitalMelbourneAustralia

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