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Haemostasis pp 335-339 | Cite as

Kaolin Clotting Time

  • Kottayam Radhakrishnan
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 992)

Abstract

The kaolin clotting time (KCT) is a sensitive test used in the laboratory detection of lupus anticoagulants (LA) (Derksen and de Groot, Thromb Res 114:521–526, 2004). It is essentially an activated partial thromboplastin time (APTT) test with no added phospholipid. Kaolin acts as the activator in the KCT. In the absence of additional phospholipid reagent, the quality of the test sample is extremely important since the generation of thrombin completely depends on the presence of residual cell membranes and plasma lipids (Derksen and de Groot, Thromb Res 114:521–526, 2004). Since the test contains no exogenous phospholipid, a confirmatory test using excess phospholipid is required to confirm the presence of lupus anticoagulant in the sample (Court, Br J Biomed Sci 54:287–298, 1997).

Key words

Kaolin clotting time (KCT) Lupus anticoagulant (LA) Activated partial thromboplastin time (APTT) Phospholipids Kaolin 

References

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    Court EL (1997) Lupus anticoagulants: pathogenesis and laboratory diagnosis. Br J Biomed Sci 54:287–298PubMedGoogle Scholar
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    Pengo V, Tripodi A, Reber G, Rand JH, Ortel L, Galli M, De Groot PG (2009) Update of the guidelines for lupus anticoagulant detection. J Thromb Haemost 7:1737–1740PubMedCrossRefGoogle Scholar
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    Tripodi A (2007) Laboratory testing for lupus anticoagulants: a review of issues affecting results. Clin Chem 53:1629–1635PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press 2013

Authors and Affiliations

  • Kottayam Radhakrishnan
    • 1
  1. 1.Department of Clinical HaematologyRoyal Children’s HospitalMelbourneAustralia

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