Advertisement

Haemostasis pp 273-277 | Cite as

Reptilase Time (RT)

  • Hratsch Karapetian
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 992)

Abstract

The reptilase time is a functional plasma clotting assay, which is based on the enzymatic activity of batroxobin. By specifically cleaving fibrinogen A from fibrinogen, batroxobin leads to the formation of a stable fibrin clot. The time, starting from the addition of batroxobin to the plasma sample, until clot formation is the reptilase time and is given in seconds. Clot formation can be detected manually or on automated coagulation systems. Reference values for healthy adults are 18–22 s. Healthy newborns may have a slightly prolonged reptilase time of up to 24 s.

In addition to other coagulation assays, the reptilase time is usually performed to confirm or to exclude the suspicion of dysfibrinogenemias. The reptilase time is independent of thrombin generation disturbances or disturbances in the action of thrombin on fibrinogen. Therefore, it can be used to confirm heparin contamination or to obtain similar information as with the thrombin clotting time in heparinized and hemophiliac patients.

Key words

Batroxobin Coagulation assay Dysfibrinogenemia Fibrin Fibrinogen Reptilase Snake venom Thrombin 

References

  1. 1.
    Aronson DL (1976) Comparison of the actions of thrombin and the thrombin-like venom enzymes ancrod and batroxobin. Thromb Haemost 36(1):9–13PubMedGoogle Scholar
  2. 2.
    Funk C, Gmur J, Herold R, Straub PW (1971) Reptilase-R—a new reagent in blood coagulation. Br J Haematol 21(1):43–52PubMedCrossRefGoogle Scholar
  3. 3.
    Barr RD, Ouna N, Simpson JG, Bagshawe AF (1976) Dysfibrinogenaemia and primary hepato-cellular carcinoma. Q J Med 45(180):647–659PubMedGoogle Scholar
  4. 4.
    Wenzel E, Holzhuter H, Muschietti F, Angelkort B, Ochs HG, Pusztai-Markos S, Nowak H, Sturner H (1974) (Reliability of tests for fibrinogen (fibrin) degradation products in plasma with thrombin coagulase, reptilase and thrombin clotting time (author’s transl)). Dtsch Med Wochenschr 99(15): 746–756PubMedCrossRefGoogle Scholar
  5. 5.
    NCCLS (1982) Tentative guidelines for the standardized, collection, transport, and preparation of blood specimens for coagulation testing and performance of coagulation assays, vol 2, no 4. NCCLS Publication, Villanova, PA, p 103–128Google Scholar
  6. 6.
    Van Cott EM, Smith EY, Galanakis DK (2002) Elevated fibrinogen in an acute phase reaction prolongs the reptilase time but typically not the thrombin time. Am J Clin Pathol 118(2):263–268PubMedCrossRefGoogle Scholar
  7. 7.
    Stocker K (1983) Reptilase-test. Thromb Res 31(5):765–766PubMedCrossRefGoogle Scholar
  8. 8.
    Capel P, Roose A, Vanderpas JB (1989) Discordance between reptilase time measured by the hook manual method and the optical method in patients with high fibrinogen level. Thromb Haemost 62(4):1143PubMedGoogle Scholar
  9. 9.
    Lefkowitz JB, DeBoom T, Weller A, Clarke S, Lavrinets D (2000) Fibrinogen longmont: a dysfibrinogenemia that causes prolonged clot-based test results only when using an optical detection method. Am J Hematol 63(3): 149–155PubMedCrossRefGoogle Scholar

Copyright information

© Humana Press 2013

Authors and Affiliations

  • Hratsch Karapetian
    • 1
  1. 1.Division of Paediatric Cardiology, Department of Paediatric and Adolescent MedicineMedical University of ViennaViennaAustria

Personalised recommendations