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Haemostasis pp 241-262 | Cite as

Platelet Flow Cytometry

  • Matthew D. Linden
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 992)

Abstract

Flow cytometry is a powerful and versatile tool which can be used to provide substantial phenotypic data on platelets by yielding quantitative information of their physical and antigenic properties. This includes surface expression of functional receptors, bound ligands, expression of granule components, interaction of platelets with other platelets via aggregation, or interaction with other blood components, such as leukocytes or the plasma coagulation system. Quantitative assessment of these parameters may facilitate the diagnosis of inherited or acquired platelet disorders, assist in the diagnosis of diseases associated with platelet activation, or assist in the monitoring of safety and efficacy of antiplatelet therapy.

Key words

Platelet activation Flow cytometry Heterotypic aggregates Platelet immunophenotyping Platelet disorders Monoclonal antibodies Fluorescence 

Notes

Acknowledgements

The author would like to thank Professor Alan D. Michelson, Asst. Prof. Andrew L. Frelinger III, and Marc R. Barnard from The Center for Platelet Research Studies at Children’s Hospital Boston for their role in the development and optimization of many of these protocols.

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Copyright information

© Humana Press 2013

Authors and Affiliations

  • Matthew D. Linden
    • 1
  1. 1.Centre for Microscopy, Characterisation and AnalysisUniversity of Western AustraliaCrawleyAustralia

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