Haemostasis pp 207-225 | Cite as

Platelet Morphology Analysis

  • Véronique Latger-Cannard
  • Odile Fenneteau
  • Sylvain Salignac
  • Thomas Pierre Lecompte
  • Nicole Schlegel
Part of the Methods in Molecular Biology book series (MIMB, volume 992)


Platelets are very small blood cells (1.5–3 μm), which play a major role in primary haemostasis and in coagulation mechanisms. Platelet characterization requires their counting (see  Chapter 15) associated with accurate morphology analysis. We describe the major steps in order to correctly obtain stained blood films, which can be analyzed by optical microscope. Platelet morphology abnormalities are found in acquired malignant hematological diseases such myeloproliferative or myelodysplastic syndromes and acute megakaryoblastic leukemia. A careful analysis of the platelet size and morphology, by detecting either normal platelets with or without excessive anisocytosis, microplatelets, or large/giant platelets, will contribute to inherited thrombocytopenia diagnosis and gather substantial data when looking for an acquired platelet disorders.

Key words

Platelets Morphology Thrombocytopenia Platelet disorders Inherited thrombocytopenia 


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Copyright information

© Humana Press 2013

Authors and Affiliations

  • Véronique Latger-Cannard
    • 1
  • Odile Fenneteau
    • 2
  • Sylvain Salignac
    • 1
  • Thomas Pierre Lecompte
    • 3
  • Nicole Schlegel
    • 2
  1. 1.Hematology Department and Grand East Competence Center on Inherited Platelet DisordersCHU NancyNancyFrance
  2. 2.Biological Hematology DepartmentNational French Reference Center on Inherited Platelet Disorders (CRPP), Robert Debré Hospital, AP-HP and Paris 7 Denis Diderot UniversityParisFrance
  3. 3.Département des Spécialités de Médecine, Service d’HématologieHôpitaux Universitaires de Genève (HUG)GenevaSwitzerland

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