Skip to main content

Fibrinogen

  • Protocol
  • First Online:
Haemostasis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 992))

Abstract

Fibrinogen is the final essential building block of the clotting process. Thus, all of the preliminary “cause and effect” events in the clotting cascade rely on the work of this molecule to measure their success. The most commonly used laboratory method for measuring fibrinogen is the Clauss fibrinogen assay. The Clauss fibrinogen assay is a quantitative, clot-based, functional assay. The assay measures the ability of fibrinogen to form fibrin clot after being exposed to a high concentration of purified thrombin. Plasma samples are pre-diluted which minimize assay interference from substances like heparin and fibrinogen degradation products. In brief, the diluted plasma is incubated at 37°C prior to the addition of the pre-warmed (37°C) thrombin reagent. From the exact moment of the addition of thrombin, the time to clot is measured. The clotting time in seconds is interpolated from a standard curve made using various dilutions of assayed standard plasma. The following chapter includes detailed information on the Clauss fibrinogen assay. Other fibrinogen assays used include fibrinogen levels derived from prothrombin time assays and antigenic methods. Fibrinogen measurements using the prothrombin time and antigenic based assays are described in brief.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Subscribe and save

Springer+ Basic
EUR 32.99 /Month
  • Get 10 units per month
  • Download Article/Chapter or Ebook
  • 1 Unit = 1 Article or 1 Chapter
  • Cancel anytime
Subscribe now

Buy Now

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Similar content being viewed by others

References

  1. Owen CA Jr (2001) Fibrinogen. In: Wl N, Bowie EJW (eds) A history of blood coagulation. Rochester, Minnesota, pp 19–26

    Google Scholar 

  2. vanCreveld S, Verder HA, Pascha CN, Kroeze WF (1959) The separation of AHF from fibrinogen. Thromb Diath Haemorrh 3:572–577

    CAS  Google Scholar 

  3. Ware AG, Guest MM, Seegers WH (1947) Fibrinogen, with special reference to its preparation and certain properties of the product. Arch Biochem 13:231–236

    PubMed  CAS  Google Scholar 

  4. Brown BA (1980) Coagulation. Hematology. Lea and Febiger, Philadelphia, pp 115–150.

    Google Scholar 

  5. Nieuwenhuizen W, Mossesson MW, De Maat MPM (2001) Fibrinogen: XVIth International Fibrinogen Workshop. Ann NY Acad Sci 645.

    Google Scholar 

  6. Gladner JA (1968) The action of fibrinogen. In: Laki K (ed) Marcel Dekker, Fibrinogen, pp 87–115

    Google Scholar 

  7. Sturge J, Carey N, Davies AH, Powell JT (2001) Fibrin monomer and fibrinopeptide B act additively to increase DNA synthesis in smooth muscle cells cultured from human saphenous vein. J Vasc Siurg 33:847–853

    Article  PubMed  CAS  Google Scholar 

  8. Owen CA Jr (2001) Inherited coagulation factor deficiencies. A history of blood coagulation. Rochester, Minnesota, p 127

    Google Scholar 

  9. Brown BA (1980) Diseases. Hematology. p 256.

    Google Scholar 

  10. Haverkate F, Samama M (1995) Familial dysfibrinogenemia and thrombophilia. Report on a study of the SSC subcommittee on fibrinogen. Thromb Haemost 73:151–161

    PubMed  CAS  Google Scholar 

  11. Mackie IJ, Kitchen S, Machin SJ, Lowe GD (2003) Guidelines on fibrinogen assays. Br J Haematol 121:396–404

    Article  PubMed  Google Scholar 

  12. Hoots WK, Carrell NA, Wagner RH, Cooper HA, McDonagh J (1981) A naturally occurring antibody that inhibits fibrin polymerization. N Engl J Med 304:857–861

    Article  PubMed  CAS  Google Scholar 

  13. Marciniak E, Greenwood MF (1979) Acquired coagulation inhibitor delaying fibrinopeptide release. Blood 53:81–92

    PubMed  CAS  Google Scholar 

  14. Rosenberg RD, Colman RW, Lorand L (1974) A new haemorrhagic disorder with defective fibrin stabilization and cryofibrinogenaemia. Br J Haematol 26:269–284

    Article  PubMed  CAS  Google Scholar 

  15. de Vries A, Rosenberg T, Kochwa S, Boss JH (1961) AM J Med 30:486–494

    Article  Google Scholar 

  16. Lowe GD, Rumley A (1999) Use of fibrinogen and fibrin D-dimer in prediction of arterial thrombotic events. Thromb Haemost 82:667–672

    PubMed  CAS  Google Scholar 

  17. Procedure for the determination of fibrinogen in plasma; approved guideline. 2nd edn. CLSI(NCCLS). 21(18):H30–A2

    Google Scholar 

  18. Clauss A (1957) Rapid physiological coagulation method in determination of fibrinogen. Acta Haematol 17:237–246

    Article  PubMed  CAS  Google Scholar 

  19. Laffen M, Manning R (2006) Investigation of haemostasis. In: Lewis SM, Bain BJ, Bates I (eds) Practical hematology. Elsevier, Philadelphia, pp 401–422

    Google Scholar 

  20. Ludlam CA, Bennett B, Fox KA, Lowe GD, Reid AW (1995) Guidelines for the use of thrombolytic therapy. Haemostasis and thrombosis task force of the British Committee for standards in haematology. Blood Coagul Fibrinolysis 6:273–285

    Article  PubMed  CAS  Google Scholar 

  21. Gosselin RC, King JH, Janatpur KA et al (2004) Effects of pentasaccharide (fondaparinux) and direct thrombin inhibitors on coagulation testing. Arch Pathol Lab Med 128:1142–1145

    PubMed  CAS  Google Scholar 

  22. Lowe GD, Rumley A, Mackie IJ (2004) Plasma fibrinogen. Ann Clin Biochem 41:430–440

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

Funding support was from the Canadian Institute of Health Research (L Mitchell Grant#114981).

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2013 Humana Press

About this protocol

Cite this protocol

Stang, L.J., Mitchell, L.G. (2013). Fibrinogen. In: Monagle, P. (eds) Haemostasis. Methods in Molecular Biology, vol 992. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-339-8_14

Download citation

  • DOI: https://doi.org/10.1007/978-1-62703-339-8_14

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-338-1

  • Online ISBN: 978-1-62703-339-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics