Haemostasis pp 131-138 | Cite as

Thrombin Clotting Time

  • Vera Ignjatovic
Part of the Methods in Molecular Biology book series (MIMB, volume 992)


Thrombin clotting time (TCT) is a coagulation assay used to diagnose congenital and acquired fibrinogen deficiency (Adcock et al., Coagulation handbook, Esoterix Coagulation, Austin, TX, 2002), as well as to identify contamination by heparin, prior to performing additional coagulation assays.

This test is based on the principle that in citrated plasma, the addition of Thrombin allows for formation of a stable clot. The time required for the formation of a stable clot is recorded in seconds and represents the actual TCT result.

Key words

TCT TT Coagulation test 


  1. 1.
    Clauss VA (1957) Gerinnungsphysiologische schnell methode zur bestimmun des fibrinogens. Acta Haematol 17:237–246PubMedCrossRefGoogle Scholar
  2. 2.
    Flanders MM, Crist R, Rodgers GM (2003) Comparison of five thrombin time reagents. Clin Chem 49:169–172PubMedCrossRefGoogle Scholar
  3. 3.
    Bockenstedt PL (1998) Laboratory methods in hemostasis. In: Loscalzo J, Schafer AI (eds) Thrombosis and hemorrhage, 2nd edn. Williams and Wilkins, Baltimore, pp 517–580Google Scholar
  4. 4.
    Ts’ao CH, Raymond J, Kolb T, Lo R (1976) Effects of source and concentration of thrombin, and divalent cations, on thrombin time of heparinized plasma. Am J Clin Pathol 65:206–212PubMedGoogle Scholar
  5. 5.
    Ray MJ, Perrin EJ, Smith IR, Hawson GA (1996) A proposed model to monitor heparin therapy using the concentrated thrombin time which allows standardisation of reagents and improved estimation of heparin concentrations. Blood Coag Fibrinol 7:515–521CrossRefGoogle Scholar
  6. 6.
    Chan A, Black L, Ing C, Williams S, Brandao L, Walter K, Abdelhaleem M (2005) Utility of aPTT in monitoring unfractionated heparin in children. J Thromb Haemost 3, abstract OR360.Google Scholar
  7. 7.
    Reid TJR, Alving BM (1993) A quantitative thrombin time for determining levels of hirudin and hirulog. Thromb Haemost 70:608–616PubMedGoogle Scholar
  8. 8.
    Shore-Lesserson L, Manspeizer HE, Bolastig M, Harrington D, Vela-Cantos F, DePerio M (2000) Anticoagulation for cardiac surgery in patients receiving preoperative heparin: use of high-dose thrombin time. Anaesth Analg 90:813–818CrossRefGoogle Scholar
  9. 9.
    Wang JS, Lin C, Karp RB (1994) Comparison of high-dose thrombin time with activated clotting time for monitoring of anticoagulant effects of heparin in cardiac surgical patients. Anaesth Analg 79:9–13Google Scholar
  10. 10.
    Love JE, Ferrell C, Chandler WL (2007) Monitoring direct thrombin inhibitors with a plasma diluted thrombin time. Thromb Haemost 98:234–242PubMedGoogle Scholar
  11. 11.
    Monagle P, Barnes C, Ignjatovic V, Furmedge J, Newall F, Chan A, De Rosa L, Hamilton S, Ragg P, Robinson S, Auldist A, Crock C, Roy N, Rowlands S (2006) Developmental haemostasis: impact for clinical haemostasis laboratories. Thromb Haemost 95:362–372PubMedGoogle Scholar
  12. 12.
    Andrew M, Paes B, Milner R, Johnston M, Mitchell L, Tollefsen DM, Powers P (1987) Development of the human coagulation system in the full-term infant. Blood 70:165–172PubMedGoogle Scholar
  13. 13.
    Andrew M, Paes B, Milner R, Jonston M, Mitchell L, Tollefsen D, Powers P (1988) Development of the human coagulation system in the healthy premature infant. Blood 72:1651–1657PubMedGoogle Scholar
  14. 14.
    Andrew M, Vegh P, Johnston M, Bowker J, Ofosu F, Mitchell L (1992) Maturation of the hemostatic system during childhood. Blood 80:1998–2005PubMedGoogle Scholar
  15. 15.
    Aronstam A, Dennis B, Friesen MN, Clark WF, Linton AL, Lindsay RM (1978) Ristocetin and the thrombin clotting time. J Clin Pathol 31:1106–1110PubMedCrossRefGoogle Scholar
  16. 16.
    NCCLS (1998) Collection, transport and processing of blood specimens for coagulation testing and general performance of coagulation assays. In: Standards NCCL (ed) 3rd edn, NCCLS, 940 West Valley Road, Wayne, PA, USA.Google Scholar
  17. 17.
    Adcock DM, Jensen R, Johns CS, Macy PA (2002) Coagulation handbook. Esoterix Coagulation, Austin, TXGoogle Scholar

Copyright information

© Humana Press 2013

Authors and Affiliations

  • Vera Ignjatovic
    • 1
    • 2
  1. 1.Haematology Research LaboratoryMurdoch Childrens Research InstituteMelbourneAustralia
  2. 2.Department of PaediatricsThe University of MelbourneMelbourneAustralia

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