Abstract
Cell-mediated nanoparticle delivery has recently emerged as an efficacious method of delivering therapeutic agents across physiological barriers. Use of cells as nanodelivery vehicles requires accurate assessment of their loading capacity and identification of intracellular compartments where nanoparticles are sequestered. This is of great interest since specific endocytic trafficking routes can ultimately influence the mode of nanoparticle release and their efficacy and function. Here, we describe a technique that allows for the isolation of individual populations of nanoparticle-containing endosomes for subsequent quantitative analysis and more accurate description of where nanoparticles are stored on a subcellular level.
Ari Nowacek and Irena Kadiu have contributed equally.
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Acknowledgments
The work was supported by the National Institutes of Health grants 1P01 DA028555, 2R01 NS034239, 2R37 NS36126, P01 NS31492, P20RR 15635, P01MH64570, and P01 NS43985 (to H.E.G.) and a research grant from Baxter Healthcare. The authors thank Ms. Robin Taylor for critical reading of the manuscript and outstanding graphic and literary support.
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Nowacek, A., Kadiu, I., McMillan, J., Gendelman, H.E. (2013). Immunoisolation of Nanoparticles Containing Endocytic Vesicles for Drug Quantitation. In: Weissig, V., Elbayoumi, T., Olsen, M. (eds) Cellular and Subcellular Nanotechnology. Methods in Molecular Biology, vol 991. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-336-7_5
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DOI: https://doi.org/10.1007/978-1-62703-336-7_5
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-335-0
Online ISBN: 978-1-62703-336-7
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