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Genotoxic Assessment of Carbon Nanotubes

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Cellular and Subcellular Nanotechnology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 991))

Abstract

Carbon nanotubes are unique one-dimensional macromolecules with promising application in biology and medicine. Since their toxicity is still under debate, here we describe an investigation of genotoxic properties of purified single-walled carbon nanotubes (SWCNT), multiwall carbon nanotubes (MWCNT), and amide-functionalized purified SWCNT. We used two different cell systems: cultured human lymphocytes where we employed cytokinesis-block micronucleus test and human fibroblasts where we investigate the induction of DNA double-strand breaks (DSBs) employing H2AX phosphorylation assay.

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Acknowledgments

This work was financially supported by the Ministry of Education and Science of the Republic of Serbia, under Project No. III 45005 and Project No. 173046.

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Authors and Affiliations

  1. Institute of Nuclear Sciences “Vinča”, University of Belgrade, Belgrade, Serbia

    Olivera Nešković, Gordana Joksić, Ana Valenta-Šobot, Jelena Cvetićanin, Djordje Trpkov, Andreja Leskovac & Sandra Petrović

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  1. Olivera Nešković
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  2. Gordana Joksić
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  3. Ana Valenta-Šobot
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  4. Jelena Cvetićanin
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  5. Djordje Trpkov
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  6. Andreja Leskovac
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  7. Sandra Petrović
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Editor information

Editors and Affiliations

  1. College of Pharmacy, Dept. Pharmaceutical Sciences, Midwestern University, N. 59th Avenue 19555, Glendale, 85308, Arizona, USA

    Volkmar Weissig

  2. , Department of Pharmaceutical Sciences, Midwestern University College of Pharmac, N. 59th Avenue 19555, Glendale, 85308, Arizona, USA

    Tamer Elbayoumi

  3. , Department of Pharmaceutical Sciences, Midwestern University College of Pharmac, N. 59th Avenue 19555, Glendale, 85308, Arizona, USA

    Mark Olsen

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Nešković, O. et al. (2013). Genotoxic Assessment of Carbon Nanotubes. In: Weissig, V., Elbayoumi, T., Olsen, M. (eds) Cellular and Subcellular Nanotechnology. Methods in Molecular Biology, vol 991. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-336-7_29

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  • DOI: https://doi.org/10.1007/978-1-62703-336-7_29

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-335-0

  • Online ISBN: 978-1-62703-336-7

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