Skin Stem Cells pp 305-313 | Cite as
Skin Stem Cells
Protocol
First Online:
- 2 Citations
- 1 Mentions
- 2.9k Downloads
Abstract
The skin stem cells are located at two distinct locations, the basement of epidermis and the hair follicle bulge. The bulge stem cell is considered to be of higher hierarchy in terms of the stemness than the epidermal stem cell. Recently, hair follicle bulge cells can be successfully isolated using fluorescence-activated cell sorting (FACS) by labeling of a specific combination of surface antigens. In this chapter, we describe a standard method by which the bulge subset in the human follicle can be isolated for subsequent functional analyses.
Key words
Skin Hair follicle Flow cytometry CD200 K15References
- 1.Cotsarelis G, Sun T-T, Lavker RM (1990) Label-retaining cells reside in the bulge of pilosebaceous unit: implications for follicular stem cells, hair cycle, and skin carcinogenesis. Cell 61:1329–1337PubMedCrossRefGoogle Scholar
- 2.Oshima H, Rochat A, Kedzia C et al (2001) Morphogenesis and renewal of hair follicles from adult multipotent stem cells. Cell 104:233–245PubMedCrossRefGoogle Scholar
- 3.Fuchs E (2009) The tortoise and the hair: slow-cycling cells in the stem cell race. Cell 137:811–819PubMedCrossRefGoogle Scholar
- 4.Rendl M, Lewis L, Fuchs E (2005) Molecular dissection of mesenchymal-epithelial interactions in the hair follicle. PLoS Biol 3:e331PubMedCrossRefGoogle Scholar
- 5.Ohyama M, Terunuma A, Tock CL et al (2006) Characterization and isolation of stem cell–enriched human hair follicle bulge cells. J Clin Invest 116:249–260PubMedCrossRefGoogle Scholar
- 6.Inoue K, Aoi N, Sato T, Yamauchi Y, Suga H, Eto H, Kato H, Araki J, Yoshimura K (2009) Differential expression of stem-cell-associated markers in human hair follicle epithelial cells. Lab Invest 89:844–856PubMedCrossRefGoogle Scholar
Copyright information
© Springer Science+Business Media New York 2013