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Isolation and Establishment of Hair Follicle Dermal Papilla Cell Cultures

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Skin Stem Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 989))

Abstract

The isolation of hair follicle dermal papilla cells has become an important technique in the field of cutaneous stem cell biology. These cells can be used for a number of biological and translational purposes. They are studied to identify the cellular characteristics and molecular factors that underpin the initiation, maintenance, and modulation of hair growth; to develop new human hair replacement techniques; and as a source of cells capable of being directed down a variety of different lineages. Here, we describe the isolation of hair follicle dermal papilla cells from both human and murine sources via the microdissection techniques used in our lab.

Karl Gledhill and Aaron Gardner have contributed equally to this work.

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Acknowledgments

The authors would like to acknowledge the photographical assistance provided by Rebecca P. Hill and Simon C. Cork in producing Figs. 1 (e–g) and 3 (respectively) and proof reading by Laura R. J. Ions. We are very grateful to DEBRA and the MRC for grant support.

Author information

Authors and Affiliations

  1. School of Biological and Biomedical Sciences, Durham University, Durham, UK

    Karl Gledhill, Aaron Gardner & Colin A. B. Jahoda

Authors
  1. Karl Gledhill
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  2. Aaron Gardner
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  3. Colin A. B. Jahoda
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Editor information

Editors and Affiliations

  1. Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Smyth Road 501, Ottawa, K1Y 8L6, Canada

    Kursad Turksen

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© 2013 Springer Science+Business Media New York

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Gledhill, K., Gardner, A., Jahoda, C.A.B. (2013). Isolation and Establishment of Hair Follicle Dermal Papilla Cell Cultures. In: Turksen, K. (eds) Skin Stem Cells. Methods in Molecular Biology, vol 989. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-330-5_22

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  • DOI: https://doi.org/10.1007/978-1-62703-330-5_22

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-329-9

  • Online ISBN: 978-1-62703-330-5

  • eBook Packages: Springer Protocols

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