Abstract
Over the last decade, science has greatly improved in the area of protein sizing and characterization. Efficient high-throughput methods are now available to substitute for the traditional labor-intensive SDS-PAGE methods, which alternatively take days to analyze a very limited number of samples. Currently, PerkinElmer® (Caliper) has designed an automated chip-based fluorescence detection method capable of analyzing proteins in minutes with sensitivity similar to standard SDS-PAGE. Here, we describe the use and implementation of this technology to characterize and screen a large number of formulations of target glycoproteins in the 14–200 kDa molecular weight range.
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Acknowledgments
The author would like to thank our colleagues in purification Bioprocess R&D for providing us with material and technical support. We also thank Yang Wang for management support.
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Chen, G., Ha, S., Rustandi, R.R. (2013). Characterization of Glycoprotein Biopharmaceutical Products by Caliper LC90 CE-SDS Gel Technology. In: Beck, A. (eds) Glycosylation Engineering of Biopharmaceuticals. Methods in Molecular Biology, vol 988. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-327-5_12
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DOI: https://doi.org/10.1007/978-1-62703-327-5_12
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-326-8
Online ISBN: 978-1-62703-327-5
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