Abstract
Primary hepatocyte cultures better reflect the properties of the liver in vivo than do cell lines derived from the liver. Here we describe a method for the isolation and culture of mouse primary hepatocytes. The cells are viable, can be transfected by DNA, and retain key properties of liver cells such as the induction of cytochrome P450 gene expression by drugs such as phenobarbital.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Wilkening S, Stahl F, Bader A (2003) Comparison of primary human hepatocytes and hepatoma cell line Hepg2 with regard to their biotransformation properties. Drug Metab Dispos 31:1035–1042
Olsavsky KM, Page JL, Johnson MC, Zarbl H, Strom SC, Omiecinski CJ (2007) Gene expression profiling and differentiation assessment in primary human hepatocyte cultures, established hepatoma cell lines, and human liver tissues. Toxicol Appl Pharmacol 222:42–56
Nyberg SL, Remmel RP, Mann HJ, Peshwa MV, Hu WS, Cerra FB (1994) Primary hepatocytes outperform Hep G2 cells as the source of biotransformation functions in a bioartificial liver. Ann Surg 220:59–67
Smirlis D, Muangmoonchai R, Edwards M, Phillips IR, Shephard EA (2001) Orphan receptor promiscuity in the induction of cytochromes P450 by xenobiotics. J Biol Chem 276:12822–12826
Klaunig JE, Goldblatt PJ, Hinton DE, Lipsky MM, Chacko J, Trump BF (1981) Mouse liver cell culture. I. Hepatocyte isolation. In Vitro 17:913–925
Bahjat FR, Dharnidharka VR, Fukuzuka K, Morel L, Crawford JM, Clare-Salzler MJ, Moldawer LL (2000) Reduced susceptibility of nonobese diabetic mice to TNF-alpha and d-galactosamine-mediated hepatocellular apoptosis and lethality. J Immunol 165:6559–6567
Page JL, Johnson MC, Olsavsky KM, Strom SC, Zarbl H, Omiecinski CJ (2007) Gene expression profiling of extracellular matrix as an effector of human hepatocyte phenotype in primary cell culture. Toxicol Sci 97:384–397
Kocarek TA, Schuetz EG, Guzelian PS (1990) Differentiated induction of cytochrome P450b/e and P450p mRNAs by dose of phenobarbital in primary cultures of adult rat hepatocytes. Mol Pharmacol 38:440–444
Acknowledgements
We thank Dr. Rick Moore (NIEHS, North Carolina, USA) for advice. L.H. is a recipient of a Drummond Scholarship.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2013 Springer Science+Business Media New York
About this protocol
Cite this protocol
Edwards, M., Houseman, L., Phillips, I.R., Shephard, E.A. (2013). Isolation of Mouse Hepatocytes. In: Phillips, I., Shephard, E., Ortiz de Montellano, P. (eds) Cytochrome P450 Protocols. Methods in Molecular Biology, vol 987. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-321-3_24
Download citation
DOI: https://doi.org/10.1007/978-1-62703-321-3_24
Published:
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-320-6
Online ISBN: 978-1-62703-321-3
eBook Packages: Springer Protocols