Abstract
As major determinants of the duration of drug action the CYP enzymes strongly influence drug efficacy and toxicity. In vivo phenotyping for CYP activities using cocktails of well-tolerated CYP-specific substrates may be valuable in the development of personalized medicine protocols, particularly for drugs that have significant toxicity profiles. However, the use of the cocktail approach in the clinic is dependent on the rapid provision of patient-specific information to the clinician. Here we describe the application of liquid chromatography–tandem mass spectrometry (LC–MS–MS) for the simultaneous phenotyping of five major drug-metabolizing CYPs in patients within a 5-min assay.
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Acknowledgement
This work was supported in part by an International Postgraduate Research Scholarship to S.G. and by the Australian National Health and Medical Research Council.
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Ghassabian, S., Murray, M. (2013). Simultaneous In Vivo Phenotyping of CYP Enzymes. In: Phillips, I., Shephard, E., Ortiz de Montellano, P. (eds) Cytochrome P450 Protocols. Methods in Molecular Biology, vol 987. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-321-3_22
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DOI: https://doi.org/10.1007/978-1-62703-321-3_22
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Publisher Name: Humana Press, Totowa, NJ
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