Skip to main content
SpringerLink
Log in
Book cover

Stem Cells and Aging pp 99–109Cite as

Isolation of Mesenchymal Stem Cells from Human Bone and Long-Term Cultivation Under Physiologic Oxygen Conditions

  • Protocol
  • First Online:

Part of the book series: Methods in Molecular Biology ((MIMB,volume 976))

Abstract

Bone-derived stroma cells contain a rare subpopulation, which exhibits enhanced stemness characteristics. Therefore, this particular cell type is often attributed the mesenchymal stem cell (MSC). Due to their high proliferation potential, multipotential differentiation capacity, and immunosuppressive properties, MSCs are now widely appreciated for cell therapeutic applications in a multitude of clinical aspects. In line with this, maintenance of MSC stemness during isolation and culture expansion is considered pivot. Here, we provide step-by-step protocols which allow selection for, and in vitro propagation of high quality MSC from human bone.

This is a preview of subscription content, log in via an institution.

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   139.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Horwitz EM, Le Blanc K, Dominici M et al (2005) Clarification of the nomenclature for MSC: the International Society for Cellular Therapy position statement. Cytotherapy 7:393–395

    Article  PubMed  CAS  Google Scholar 

  2. Valtieri M, Sorrentino A (2008) The mesenchymal stromal cell contribution to homeostasis. J Cell Physiol 217:296–300

    Article  PubMed  CAS  Google Scholar 

  3. Phinney DG, Prockop DJ (2007) Concise review: mesenchymal stem/multipotent stromal cells: the state of transdifferentiation and modes of tissue repair—current views. Stem Cells 25:2896–2902

    Article  PubMed  Google Scholar 

  4. Gamblin SJ, Davies GJ, Grimes JM et al (1991) Activity and specificity of human aldolases. J Mol Biol 219:573–576

    Article  PubMed  CAS  Google Scholar 

  5. Crisan M, Yap S, Casteilla L et al (2008) A perivascular origin for mesenchymal stem cells in multiple human organs. Cell Stem Cell 3:301–313

    Article  PubMed  CAS  Google Scholar 

  6. Mendez-Ferrer S, Michurina TV, Ferraro F et al (2010) Mesenchymal and haematopoietic stem cells form a unique bone marrow niche. Nature 466:829–834

    Article  PubMed  CAS  Google Scholar 

  7. D’Ippolito G, Diabira S, Howard GA et al (2004) Marrow-isolated adult multilineage inducible (MIAMI) cells, a unique population of postnatal young and old human cells with extensive expansion and differentiation potential. J Cell Sci 117:2971–2981

    Article  PubMed  Google Scholar 

  8. Fehrer C, Brunauer R, Laschober G et al (2007) Reduced oxygen tension attenuates differentiation capacity of human mesenchymal stem cells and prolongs their lifespan. Aging Cell 6:745–757

    Article  PubMed  CAS  Google Scholar 

  9. Gronthos S, Graves SE, Ohta S et al (1994) The STRO-1+ fraction of adult human bone marrow contains the osteogenic precursors. Blood 84:4164–4173

    PubMed  CAS  Google Scholar 

  10. Peister A, Mellad JA, Larson BL et al (2004) Adult stem cells from bone marrow (MSCs) isolated from different strains of inbred mice vary in surface epitopes, rates of proliferation, and differentiation potential. Blood 103:1662–1668

    Article  PubMed  CAS  Google Scholar 

  11. Friedenstein AJ, Petrakova KV, Kurolesova AI et al (1968) Heterotopic of bone marrow. Analysis of precursor cells for osteogenic and hematopoietic tissues. Transplantation 6:230–247

    Article  PubMed  CAS  Google Scholar 

  12. Dominici M, Le Blanc K, Mueller I et al (2006) Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement. Cytotherapy 8:315–317

    Article  PubMed  CAS  Google Scholar 

  13. Bianco P, Robey PG, Simmons PJ (2008) Mesenchymal stem cells: revisiting history, concepts, and assays. Cell Stem Cell 2:313–319

    Article  PubMed  CAS  Google Scholar 

  14. Simonsen JL, Rosada C, Serakinci N et al (2002) Telomerase expression extends the proliferative life-span and maintains the osteogenic potential of human bone marrow stromal cells. Nat Biotechnol 20:592–596

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgment

SR is supported by a Marie Curie International Reintegration Grant. GL is supported by research funds granted by the Austrian Research Agency FFG—Laura Bassi Centre of Expertise DIALIFE, the Translational Research Programme of the Tyrolean Future Fund, and the EC’s 7th framework program VascuBone FP7-HEALTH-2009-single-stage-242175 coordinated by Heike Walles, University of Würzburg.

Author information

Authors and Affiliations

  1. The Extracellular Matrix Research Group, Institute for Biomedical Aging Research, Austrian Academy of Sciences, Innsbruck, Austria

    Sebastian Klepsch, Angelika Jamnig, Daniela Trimmel, Magdalena Schimke, Werner Kapferer, Regina Brunauer, Sarvpreet Singh, Stephan Reitinger & Günter Lepperdinger

Authors
  1. Sebastian Klepsch
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Angelika Jamnig
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Daniela Trimmel
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Magdalena Schimke
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Werner Kapferer
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Regina Brunauer
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Sarvpreet Singh
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Stephan Reitinger
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Günter Lepperdinger
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Günter Lepperdinger .

Editor information

Editors and Affiliations

  1. Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Smyth Road 501, Ottawa, K1Y 8L6, Canada

    Kursad Turksen

Rights and permissions

Reprints and permissions

Copyright information

© 2013 Springer Science+Business Media, LLC

About this protocol

Cite this protocol

Klepsch, S. et al. (2013). Isolation of Mesenchymal Stem Cells from Human Bone and Long-Term Cultivation Under Physiologic Oxygen Conditions. In: Turksen, K. (eds) Stem Cells and Aging. Methods in Molecular Biology, vol 976. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-317-6_8

Download citation

  • DOI: https://doi.org/10.1007/978-1-62703-317-6_8

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-316-9

  • Online ISBN: 978-1-62703-317-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation