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Stem Cells and Aging pp 1–15Cite as

Comprehensive Hematopoietic Stem Cell Isolation Methods

Part of the Methods in Molecular Biology book series (MIMB,volume 976)

Abstract

The use of flow cytometry has been critical in establishing methods to isolate and characterize hematopoietic stem cells (HSCs) and their progenitors. For more than 30 years, researchers have been uncovering novel markers that when used in combination significantly enhance the purification of HSCs from murine and human bone marrow. The complex interface between HSCs, the lymphohematopoietic system, and their niches, has made identification of HSC markers critical to understanding their biological nature, more so than other adult stem cell populations. Here we review the phenotypic markers and strategies used to purify HSCs, the appropriateness of using these markers for comparisons of HSC function at different stages of ontogeny, and their utility in defining the lineage bias in the HSC compartment.

Keywords

  • Hematopoietic stem cells
  • Isolation methods
  • Phenotypic markers
  • Lineage bias

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Acknowledgments

We would like to thank Paula Thomason for her expert editorial assistance in the preparation of the manuscript and Ying Liang for her critical review.

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Authors and Affiliations

  1. Departments of Physiology, Markey Cancer Center, University of Kentucky, Lexington, KY, USA

    Kyle Rector & Gary Van Zant

  2. Department of Physiology, Markey Cancer Center, University of Kentucky, Lexington, KY, USA

    Yi Liu

Authors
  1. Kyle Rector
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  2. Yi Liu
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  3. Gary Van Zant
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Corresponding author

Correspondence to Kyle Rector .

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Editors and Affiliations

  1. Sprott Centre for Stem Cell Research, Regenerative Medicine Program, Ottawa Hospital Research Institute, Smyth Road 501, Ottawa, K1Y 8L6, Canada

    Kursad Turksen

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Rector, K., Liu, Y., Van Zant, G. (2013). Comprehensive Hematopoietic Stem Cell Isolation Methods. In: Turksen, K. (eds) Stem Cells and Aging. Methods in Molecular Biology, vol 976. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-317-6_1

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  • DOI: https://doi.org/10.1007/978-1-62703-317-6_1

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-316-9

  • Online ISBN: 978-1-62703-317-6

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