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In Vivo Angiogenic Activity of Erythropoietin

  • Domenico Ribatti
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 982)

Abstract

The role of erythropoietin (Epo) has been demonstrated in tissues outside the hematopoietic system, including the cardiovascular system, where Epo promotes various effects in endothelial cells. Here, we have demonstrated the angiogenic capacity of recombinant human Epo (rhuEpo) in vivo, by means of the chick embryo chorioallantoic membrane (CAM) assay, a well-established in vivo assay to study angiogenesis and antiangiogenesis.

Key words

Angiogenesis Chorioallantoic membrane Erythropoietin 

Notes

Acknowledgement

Supported by European Seventh Framework Programme (FTP/2007-2013) under grant agreement No 278570 to DR.

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Domenico Ribatti
    • 1
  1. 1.Department of Basic Medical Sciences, Neurosciences, and Sensory OrgansUniversity of Bari Medical SchoolBariItaly

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