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Dictyostelium discoideum Protocols pp 93–112Cite as

Fluorescent Reporters and Methods to Analyze Fluorescent Signals

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 983))

Abstract

The use of fluorescent reporters and the development of new imaging technologies have revolutionized studies in cell biology. During recent years the number of fluorescent proteins offering the ability to visualize the distribution of proteins, organelles, and cells has increased tremendously. In parallel, the imaging tools available were refined rapidly enabling now the use of a huge spectrum of specialized methods to explore the cellular and subcellular localization and dynamics of fluorescently tagged markers. This chapter presents an overview of fluorescent reporters and methods available, and describes a selection of those that are routinely applicable in imaging studies using Dictyostelium discoideum.

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Acknowledgment

This work was supported by DFG grants SFB 863 and SFB 914.

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Authors and Affiliations

  1. Institut für Anatomie und Zellbiologie, Ludwig-Maximilians-Universität München, Munich, Germany

    Annette Müller-Taubenberger & Hellen C. Ishikawa-Ankerhold

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  1. Annette Müller-Taubenberger
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  2. Hellen C. Ishikawa-Ankerhold
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Correspondence to Annette Müller-Taubenberger .

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Editors and Affiliations

  1. , Centre for Biochemistry, University of Cologne, Joseph-Stelzmann-Str. 52, Köln, 50931, Nordrhein-Westfalen, Germany

    Ludwig Eichinger

  2. , Centre for Biomedical Research, University of Hull, Hull, HU6 7RX, Montserrat

    Francisco Rivero

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Müller-Taubenberger, A., Ishikawa-Ankerhold, H.C. (2013). Fluorescent Reporters and Methods to Analyze Fluorescent Signals. In: Eichinger, L., Rivero, F. (eds) Dictyostelium discoideum Protocols. Methods in Molecular Biology, vol 983. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-302-2_5

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  • DOI: https://doi.org/10.1007/978-1-62703-302-2_5

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