Evaluating Amyloid Beta (Aβ) 1-40 Degradation by Capillary Electrophoresis
Here, we describe a capillary electrophoresis method for evaluating proteolysis of the amyloid beta peptide (Aβ) 1–40. This method is suitable for kinetic studies, demands little specialized equipment, and consumes only small quantities of a commercially available substrate whose physiological accumulation is thought to underlie the development of Alzheimer’s disease.
Key wordsCapillary electrophoresis Amyloid beta (Aβ) 1–40 Proteolysis Insulin-degrading enzyme
We are grateful to Drs. Nandu Menon, Alan Przybyla, and Zachary Wood (all of the University of Georgia, Department of Biochemistry and Molecular Biology) for advice and assistance, and for access to experimental equipment and materials. Aβ 1–40 consumed during method development and analysis was a gift of rPeptide, Inc. (Athens, GA, USA).