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Contribution of CE to the Analysis of Protein or Peptide Biomarkers

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 984)

Abstract

Biomarker analysis is pivotal for disease diagnosis and one important class of biomarkers is constituted by proteins and peptides. This review focuses on protein and peptide analyses from biological fluids performed by capillary electrophoresis. The various strategies that have been reported to prevent difficulties due to the handling of real samples are described. Innovative techniques to overcome the complexity of the sample, to prevent the adsorption of the analytes on the inner capillary wall, and to increase the sensibility of the analysis are summarized and illustrated by different applications. To fully illustrate the contribution of CE to the analysis of biomarkers from human sample, two detailed protocols are given: the analysis from CSF of five amyloid peptide, biomarkers of the Alzheimer disease, and the analysis of sialoforms of transferrin from human serum.

Key words

Capillary electrophoresis Biomarkers Coating Preconcentration Biological fluids 
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Notes

Acknowledgments

This review’s writing was supported by the European Union’s NADINE project under Contract No. 246513. François de l’Escaille and Jean-Bernard Falmagne are employees of Analis s.a. Belgium. Analis s.a. is producer of CEofix™ NTMP and CEofix™ CDT kits and editor of the PHoEBuS software.

References

  1. 1.
    Atkinson AJ, Wayne A et al (2001) Biomarkers and surrogate endpoints: preferred definitions and conceptual framework. Clin Pharmacol Ther 69:89–95CrossRefGoogle Scholar
  2. 2.
    Chen R, Jin Z, Colón LA (1996) Analysis of tear fluid by CE/LIF: a noninvasive approach for glucose monitoring. J Capillary Electrophor 3:243–248PubMedGoogle Scholar
  3. 3.
    Tomosugi N et al (2005) Diagnostic potential of tear proteomic patterns in Sjögren’ssyndrome. J Proteome Res 4:820–825PubMedCrossRefGoogle Scholar
  4. 4.
    Zhou L et al (2009) Identification of tear fluid biomarkers in dry eye syndrome using iTRAQ quantitative proteomics. J Proteome Res 8:4889–4905PubMedCrossRefGoogle Scholar
  5. 5.
    Van Eijk HMH et al (1999) Automated isolation of high-purity plasma albumin for isotope ratio measurements. J Chromatogr B 731:199–205CrossRefGoogle Scholar
  6. 6.
    Kakehi K et al (2001) Capillary electrophoresis of sialic acid-containing glycoprotein. Effect of the heterogeneity of carbohydrate chains on glycoform separation using an alpha 1-acid glycoprotein as a model. Anal Chem 73:2640–2647PubMedCrossRefGoogle Scholar
  7. 7.
    Valcárcel M, Arce L, Ríos A (2001) Coupling continuous separation techniques to capillary electrophoresis. J Chromatogr A 924:3–30PubMedCrossRefGoogle Scholar
  8. 8.
    Guzman NA, Trebilcock MA, Advis JP (1990) Paper presented at the First Annual Conference on Capillary Electrophoresis. Frederick, Maryland, October 15–16, Abstract no. 12.Google Scholar
  9. 9.
    Guzman NA, Trebilcock MA, Advis JP (1991) The Use of a Concentration Step to Collect Urinary Components Separated by Capillary Electrophoresis and Further Characterization of Collected Analytes by Mass Spectrometry. J Liq Chromatogr 14:997–1015CrossRefGoogle Scholar
  10. 10.
    Dalluge J, Sander LC (1998) Precolumn Affinity Capillary Electrophoresis for the Identification of Clinically Relevant Proteins in Human Serum: Application to Human Cardiac Troponin I. Anal Chem 70:5339–5343PubMedCrossRefGoogle Scholar
  11. 11.
    Peoples MC, Karnes HT (2008) Microfluidic Capillary System for Immunoaffinity Separations of C-Reactive Protein in Human Serum and Cerebrospinal Fluid. Anal Chem 80:3853–3858PubMedCrossRefGoogle Scholar
  12. 12.
    Yang YZ, Boysen RI, Hearn MTW (2006) Optimization of field-amplified sample injection for analysis of peptides by capillary electrophoresis−mass spectrometry. Anal Chem 78:4752–4758PubMedCrossRefGoogle Scholar
  13. 13.
    Armenta J et al (2007) Coupled affinity-hydrophobic monolithic column for on-line removal of immunoglobulin G. preconcentrated of low abundance proteins and separation by capillary zone electrophoresis. J Chromatogr A 1148:115–122PubMedCrossRefGoogle Scholar
  14. 14.
    Kaiser T et al (2004) Capillary electrophoresis coupled to mass spectrometer for automated and robust polypeptide determination in body fluids for clinical use. Electrophoresis 25:2044–2055PubMedCrossRefGoogle Scholar
  15. 15.
    Ongay S et al (2010) Development of a fast and simple immunochromatographic method to purify alpha 1-acid glycoprotein from serum for analysis of its isoforms by capillary electrophoresis. Anal Chim Acta 663:206–212PubMedCrossRefGoogle Scholar
  16. 16.
    Li XM, Zhang F, Zhang SS (2008) Capillary electrophoresis enzyme immunoassay for alpha-fetoprotein and thyroxine in human serum with electrochemical detection. J Sep Sci 31:336–340PubMedCrossRefGoogle Scholar
  17. 17.
    Guzman NA et al (2003) Improved solid-phase microextraction device for use in on-line immunoaffinity capillary electrophoresis. Electrophoresis 24:3718–3727PubMedCrossRefGoogle Scholar
  18. 18.
    Amundsen LK, Sirén H (2007) Immunoaffinity CE in clinical analysis of body fluids and tissues. Electrophoresis 28:99–113PubMedCrossRefGoogle Scholar
  19. 19.
    Van der Veen M, Norde W, Stuart MC (2004) Electrostatic interactions in protein adsorption probed by comparing lysozyme and succinylated lysozyme. Colloids Surf B Biointerfaces 35:33–40PubMedCrossRefGoogle Scholar
  20. 20.
    Gray JJ et al (2004) the interaction of proteins with solid surfaces. Curr. Opin. Struct. Biol 14:110–115Google Scholar
  21. 21.
    Malmsten M et al (1998) Formation of Adsorbed Protein Layers. J Colloid Interface Sci 207:186–199PubMedCrossRefGoogle Scholar
  22. 22.
    Ding HM et al (2005) Silica nanotubes for lysozyme immobilization. J Colloid Interface Sci 290:102–106PubMedCrossRefGoogle Scholar
  23. 23.
    Hamrníkova I et al (1999) Binding of proline- and hydroxyproline-containing peptides and proteins to the capillary wall. J Chromatogr A 838:167–177CrossRefGoogle Scholar
  24. 24.
    Verzola B, Gelfi C, Righetti PG (2000) Protein adsorption to the bare silica wall in capillary electrophoresis quantitative study on the chemical composition of the background electrolyte for minimising the phenomenon. J Chromatogr A 868:85–99PubMedCrossRefGoogle Scholar
  25. 25.
    Nakanishi K, Sakiyama T, Imamura K (2001) On the adsorption of proteins on solid surfaces, a common but very complicated phenomenon. J BiosciBioeng 91:233–244Google Scholar
  26. 26.
    Essa H et al (2007) Influence of pH and ionic strength on the adsorption, leaching and activity of myoglobin immobilized onto ordered mesoporoussilicates. J Mol Catal Enzym 49:61–68CrossRefGoogle Scholar
  27. 27.
    Lucy CA, MacDonald AM, Gulcev MD (2008) Non-covalent capillary coatings for protein separations in capillary electrophoresis. J Chromatogr A 1184:81–105PubMedCrossRefGoogle Scholar
  28. 28.
    Stutz H (2009) Protein attachment onto silica surfaces–a survey of molecular fundamentals, resulting effects and novel preventive strategies in CE. Electrophoresis 30:2032–2061PubMedCrossRefGoogle Scholar
  29. 29.
    Weinbauer M, Stutz H (2010) Successive multiple ionic polymer layer coated capillaries in the separation of proteins - Recombinant allergen variants as a case study. Electrophoresis 31:1805–1812PubMedCrossRefGoogle Scholar
  30. 30.
    Giordano BC et al (2000) Dynamically-coated capillaries allow for capillary electrophoretic resolution of transferrin sialoforms via direct analysis of human serum. J Chromatogr B 742:79–89CrossRefGoogle Scholar
  31. 31.
    Lanz C et al (2002) Evaluation and optimization of capillary zone electrophoresis with different dynamic capillary coatings for the determination of carbohydrate-deficient transferrin in human serum. J Chromatogr A 979:43–57PubMedCrossRefGoogle Scholar
  32. 32.
    Yang R, Liu Y, Wang Y (2009) Hydroxyethylcellulose-graft-poly (4-vinylpyridine) as a novel, adsorbed coating for protein separation by capillary electrophoresis. Electrophoresis 30:2321–2327PubMedCrossRefGoogle Scholar
  33. 33.
    Sassi AP et al (2005) An automated, sheathless capillary electrophoresis-mass spectrometry platform for discovery of biomarkers in human serum. Electrophoresis 26:1500–1512PubMedCrossRefGoogle Scholar
  34. 34.
    Liu H et al (2008) A well-defined diblock copolymer of poly-(ethylene oxide)-block-poly (4-vinylpyridine) for separation of basic proteins by capillary zone electrophoresis. Electrophoresis 29:2812–2819PubMedGoogle Scholar
  35. 35.
    Puerta A et al (2006) Novel adsorptive polyamine coating for enhanced capillary electrophoresis of basic proteins and peptides. J Chromatogr B 838:113–121CrossRefGoogle Scholar
  36. 36.
    Isemura T, Kitagawa F, Otsuka K (2009) Separation of complex mixtures of fluorobenzoic acids by capillary electrophoresis. J Sep Sci 32:381–387PubMedCrossRefGoogle Scholar
  37. 37.
    Mischak H, Schanstra JP (2011) CE-MS in biomarker discovery, validation, and clinical application. Proteomics Clin Appl 5:9–23PubMedCrossRefGoogle Scholar
  38. 38.
    Szökő E, Tábi T (2010) Analysis of biological samples by capillary electrophoresis with laser induced fluorescence detection. J Pharm Biomed Anal 53:1180–1192PubMedCrossRefGoogle Scholar
  39. 39.
    Breadmore MC et al (2011) Recent advances in enhancing the sensitivity of electrophoresis and electrochromatography in capillaries and microchips (2008–2010). Electrophoresis 32:127–148PubMedCrossRefGoogle Scholar
  40. 40.
    Mala Z et al (2011) Contemporary sample stacking in analytical electrophoresis. Electrophoresis 32:116–126PubMedCrossRefGoogle Scholar
  41. 41.
    Chen Y et al (2009) Assay of bradykinin metabolites in human body fluids by CE-LIF coupled with transient ITP preconcentration. Electrophoresis 30:2300–2306PubMedCrossRefGoogle Scholar
  42. 42.
    Yang WC, Yeung ES, Schmerr MJ (2005) Detection of prion protein using a capillary electrophoresis-based competitive immunoassay with laser-induced fluorescence detection and cyclodextrin-aided separation. Electrophoresis 26:1751–1759PubMedCrossRefGoogle Scholar
  43. 43.
    Babu S, Chung BC, Lho DS, Yoo YS (2006) Capillary electrophoretic competitive immunoassay with laser-induced fluorescence detection for methionine-enkephalin. JChromatogr 1111:133–138CrossRefGoogle Scholar
  44. 44.
    Verpillot R et al (2011) Analysis of amyloid-β peptides in cerebrospinal fluid samples by capillary electrophoresis coupled with LIF detection. AnalChem 83:1696–1703Google Scholar
  45. 45.
    Tu J et al (2003) Application of multiplexed capillary electrophoresis with laser-induced fluorescence (MCE-LIF) detection for the rapid measurement of endogenous extracellular signal-regulated protein kinase (ERK) levels in cell extracts. J Chromatogr B 789:323–335CrossRefGoogle Scholar
  46. 46.
    Chen Y, Xu L, Lin J, Chen G (2008) Assay of bradykinin-related peptides in human body fluids using capillary electrophoresis with laser-induced fluorescence detection. Electro-phoresis 29:1302–1307PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.Laboratory of Proteins and Nanotechnologies in Separation Sciences, Faculté de PharmacieUniversity of Paris-Sud, UMR-CNRS 8612Châtenay-MalabryFrance
  2. 2.R&D DIAG, Analis SASuarléeBelgium

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