Abstract
A new method for protein sequence diversification is based on generating random codon mutations to an encoding DNA. This allows for the scanning of user-defined amino acid changes to any protein of interest, and is an alternative to traditional directed evolution strategies. This chapter describes the procedures required to apply this technology to any protein of interest. The resulting libraries can then be screened for new or improved protein function.
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Acknowledgments
The authors thank the National Institutes of Health (GM084396) for financial support.
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Liu, J., Cropp, T.A. (2013). Rational Protein Sequence Diversification by Multi-Codon Scanning Mutagenesis. In: Samuelson, J. (eds) Enzyme Engineering. Methods in Molecular Biology, vol 978. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-293-3_16
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DOI: https://doi.org/10.1007/978-1-62703-293-3_16
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-292-6
Online ISBN: 978-1-62703-293-3
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