Abstract
The low frequency of T cells specific for given antigens makes the study of antigen-specific T cell responses difficult. The development of MHC class I and II tetramer staining techniques allows precise quantification and tracking of antigen-specific CD8+ and CD4+ T cell responses. Here, we describe a protocol for MHC class I and II tetramer staining of mouse T cells isolated from various tissues of mice infected with lymphocytic choriomeningitis virus (LCMV) or with murine cytomegalovirus (MCMV).
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Acknowledgments
This work was supported by Public Health Service Grants AI057753, DK081175 (D.A.H.).
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Kurtulus, S., Hildeman, D. (2013). Assessment of CD4+ and CD8+ T Cell Responses Using MHC Class I and II Tetramers. In: Snow, A., Lenardo, M. (eds) Immune Homeostasis. Methods in Molecular Biology, vol 979. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-290-2_8
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DOI: https://doi.org/10.1007/978-1-62703-290-2_8
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Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-1-62703-289-6
Online ISBN: 978-1-62703-290-2
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