Methods for Studies of Protein Interactions with Different DNA Methyltransferases
There are now many methods available for studying protein interactions between DNA methltransferases (DNMTs) and their binding partners. Here we describe a step-by-step procedure to identify whether proteins of interest interact with DNMTs by co-immunoprecipitation (co-IP) assay in transiently transfected cells. Though one mammalian cell is described, investigators can use the same method with other cell lines or primary cells for in vivo protein interaction studies.
Key wordsProtein interactions DNA methyltransferases Co-immunoprecipitation Transfection Epitope tag SALL4 Cytosine methylation
This work was supported by the American Cancer Society-Institutional Research Grant and the Leukemia & Lymphoma Society Special Fellow award (3366-09).
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