Mammalian Two-Hybrid Assays for Studies of Interaction of p300 with Transcription Factors

  • Daniela B. Mendonça
  • Gustavo Mendonça
  • Lyndon F. Cooper
Part of the Methods in Molecular Biology book series (MIMB, volume 977)


The two-hybrid system is a powerful genetic assay that allows the interaction between two proteins to be detected in vivo. It was originally described in 1989 and since then it has been one of the main techniques used to identify interactions between proteins from different cellular organisms. Here we describe the methods to study the interaction of p300 with other transcription factors, specifically between p300 and two transcription factors related to hypoxia and inflammation, HIF-1α and NF-κB-p65, respectively.

Key words

Mammalian two-hybrid p300 Protein–protein interaction Gal4 VP16 Transcription 


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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Daniela B. Mendonça
    • 1
  • Gustavo Mendonça
    • 1
  • Lyndon F. Cooper
    • 1
  1. 1.Bone Biology and Implant Therapy Laboratory, Department of ProsthodonticsUniversity of North Carolina at Chapel HillChapel HillUSA

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