Preparation of Cell Lines for Single-Cell Analysis of Transcriptional Activation Dynamics

  • Ilona U. Rafalska-Metcalf
  • Susan M. Janicki
Part of the Methods in Molecular Biology book series (MIMB, volume 977)


Imaging molecularly defined regions of chromatin in single living cells during transcriptional activation has the potential to provide new insight into gene regulatory mechanisms. Here, we describe a method for isolating cell lines with multi-copy arrays of reporter transgenes, which can be used for real-time high-resolution imaging of transcriptional activation dynamics in single cells.

Key words

Live-cell imaging Transgene array Stable-cell lines Transcription Chromatin 


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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Ilona U. Rafalska-Metcalf
    • 1
  • Susan M. Janicki
    • 1
  1. 1.Molecular and Cellular Oncogenesis ProgramThe Wistar InstitutePhiladelphiaUSA

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