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Nuclear Recruitment Assay as a Tool to Validate Transcription Factor Interactions in Mammalian Cells

  • C. J. J. Boogerd
  • V. M. Christoffels
  • P. Barnett
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 977)

Abstract

Identification and verification of novel transcription factor interactions is an inherent step in the discovery of molecular mechanisms driving gene transcription and regulation. Co-immunoprecipitation and GST-pull down are often key techniques in the verification process. Despite wide applicability, their use may sometimes be restricted. We provide a detailed protocol for an intracellular immunofluorescence technique that may be used as an alternative or complimentary study for transcription factor interaction verification.

Key words

Transcription factor Transfection Nuclear import Interaction Immunofluorescence 

Notes

Acknowledgments

This work was supported by European Community’s Sixth Frame­work Programme contract HeartRepair LSHM-CT-2005-018630.

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • C. J. J. Boogerd
    • 1
  • V. M. Christoffels
    • 2
  • P. Barnett
    • 2
  1. 1.Heart Failure Research Center, Academic Medical Center, section K2AmsterdamThe Netherlands
  2. 2.Heart Failure Research Centre, Academic Medical Centre, section K2AmsterdamThe Netherlands

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