Abstract
The chiral separation principle ligand-exchange is applicable for HPLC, capillary electrophoresis as well as for capillary electrochromatography. Among other chiral separation principles, ligand-exchange permits simple method optimization and can be used particularly for chiral amino acids, amino alcohols or α-hydroxy acids. Up to now, three different approaches how to prepare capillaries for ligand-exchange electrochromatography have been published: The use of packed columns, monolithic columns, and particle-loaded columns. This chapter describes examples for the preparation of such columns for all three approaches and illustrates their use for chiral separations.
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Abbreviations
- CEC:
-
Capillary electrochromatography
- CSP:
-
Chiral stationary phase
- EEO:
-
Enantiomer elution order
- EOF:
-
Electroosmotic flow
- LE:
-
Ligand-exchange
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Schmid, M.G. (2013). Application of Chiral Ligand-Exchange Stationary Phases in Capillary Electrochromatography. In: Scriba, G. (eds) Chiral Separations. Methods in Molecular Biology, vol 970. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-263-6_28
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DOI: https://doi.org/10.1007/978-1-62703-263-6_28
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