Skip to main content

APEX DNA Microarray for the Identification of Pathogenic Fungi

  • Protocol
  • First Online:
Fungal Diagnostics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 968))

Abstract

The identification of fungal pathogens, though continuously improving, is still time-consuming and often inadequate for ensuring an early targeted therapy, which may be crucial for the treatment of invasive mycoses. Here, we describe a DNA-microarray system based on the arrayed-primer extension (APEX) technique for a rapid identification of pathogenic fungi, which represents a critical step in medical practice.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 119.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 169.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Landlinger C, Preuner S, Willinger B et al (2009) Species-specific identification of a wide range of clinically relevant fungal pathogens by use of Luminex xMAP technology. J Clin Microbiol 47:1063–1073

    Article  PubMed  CAS  Google Scholar 

  2. Campa D, Tavanti A, Gemignani F et al (2008) DNA microarray based on arrayed-primer extension technique for identification of pathogenic fungi responsible for invasive and superficial mycoses. J Clin Microbiol 46:909–915

    Article  PubMed  CAS  Google Scholar 

  3. White T, Bruns T, Lee S et al (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis M, Gelfand D, Sninsky J, White T (eds) PCR protocols: a guide to methods and application. Academic Press, San Diego, CA, pp 315–322

    Google Scholar 

  4. Gemignani F, Landi S, Chabrier A et al (2004) Generation of a DNA microarray for determination of E6 natural variants of human papillomavirus type 16. J Virol Methods 119:95–102

    Article  PubMed  CAS  Google Scholar 

  5. Gemignani F, Perra C, Landi S et al (2002) Reliable detection of beta-thalassemia and G6PD mutations by a DNA microarray. Clin Chem 48:2051–2054

    PubMed  CAS  Google Scholar 

  6. Landi S, Gemignani F, Gioia-Patricola L et al (2003) Evaluation of a microarray for genotyping polymorphisms related to xenobiotic metabolism and DNA repair. Biotechniques 35:816–827

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by research grant 200985J87J_003 from the “Ministero dell’Istruzione, dell’Università e della Ricerca” (MIUR) and by an “Associazione Italiana Ricerca sul Cancro” (AIRC) regional grant.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Sonia Senesi .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2013 Springer Science+Business Media New York

About this protocol

Cite this protocol

Tavanti, A., Landi, S., Senesi, S. (2013). APEX DNA Microarray for the Identification of Pathogenic Fungi. In: O'Connor, L., Glynn, B. (eds) Fungal Diagnostics. Methods in Molecular Biology, vol 968. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-257-5_4

Download citation

  • DOI: https://doi.org/10.1007/978-1-62703-257-5_4

  • Published:

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-256-8

  • Online ISBN: 978-1-62703-257-5

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics