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Dopamine pp 15-24 | Cite as

Methods for the Study of Dopamine Receptors Within Lipid Rafts of Kidney Cells

  • Peiying Yu
  • Van Anthony Villar
  • Pedro A. JoseEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 964)

Abstract

There is increasing evidence that G protein-coupled receptor (GPCR) signaling is regulated in lipid raft microdomains. GPCRs and GPCR-signaling molecules, including G proteins and protein kinases, have been reported to compartmentalize in these microdomains. Dopamine D1-like receptors (D1R and D5R) belong to a family of GPCRs that are important in the regulation of renal function. These receptors are not only localized and regulated in caveolae that contains caveolin-1 but are also distributed in non-­caveolar lipid rafts which do not contain caveolin-1. This chapter describes detergent- and non-detergent-based methods to obtain lipid raft fractions from renal proximal tubule cells.

Key words

Lipid rafts Caveolae Membrane microdomains Dopamine receptor 

Notes

Acknowledgments

These studies were supported in part by grants from the National Institutes of Health (HL023081, HL074940, DK039308, HL068686, and HL092196).

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Peiying Yu
    • 1
  • Van Anthony Villar
    • 2
  • Pedro A. Jose
    • 2
    Email author
  1. 1.Department of Pediatrics, Center for Molecular Physiology Research, Children’s National Medical Center, School of MedicineGeorge Washington UniversityWashingtonUSA
  2. 2.Department of Pediatrics, Center for Molecular Physiology Research, Children’s National Medical Center, and School of MedicineGeorge Washington UniversityWashingtonUSA

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