Methods for the Study of Dopamine Receptors Within Lipid Rafts of Kidney Cells
There is increasing evidence that G protein-coupled receptor (GPCR) signaling is regulated in lipid raft microdomains. GPCRs and GPCR-signaling molecules, including G proteins and protein kinases, have been reported to compartmentalize in these microdomains. Dopamine D1-like receptors (D1R and D5R) belong to a family of GPCRs that are important in the regulation of renal function. These receptors are not only localized and regulated in caveolae that contains caveolin-1 but are also distributed in non-caveolar lipid rafts which do not contain caveolin-1. This chapter describes detergent- and non-detergent-based methods to obtain lipid raft fractions from renal proximal tubule cells.
Key wordsLipid rafts Caveolae Membrane microdomains Dopamine receptor
These studies were supported in part by grants from the National Institutes of Health (HL023081, HL074940, DK039308, HL068686, and HL092196).