Dopamine pp 243-273 | Cite as

Monitoring Axonal and Somatodendritic Dopamine Release Using Fast-Scan Cyclic Voltammetry in Brain Slices

  • Jyoti C. PatelEmail author
  • Margaret E. Rice
Part of the Methods in Molecular Biology book series (MIMB, volume 964)


Brain dopamine pathways serve wide-ranging functions including the control of movement, reward, cognition, learning, and mood. Consequently, dysfunction of dopamine transmission has been implicated in clinical conditions such as Parkinson’s disease, schizophrenia, addiction, and depression. Establishing factors that regulate dopamine release can provide novel insights into dopaminergic communication under normal conditions, as well as in animal models of disease in the brain. Here we describe methods for the study of somatodendritic and axonal dopamine release in brain slice preparations. Topics covered include preparation and calibration of carbon-fiber microelectrodes for use with fast-scan cyclic voltammetry, preparation of midbrain and forebrain slices, and procedures of eliciting and recording electrically evoked dopamine release from in vitro brain slices.

Key words

Dopamine Brain slices Voltammetry Carbon-fiber microelectrodes Striatum Substantia nigra pars compacta Ventral tegmental area 



We are grateful for support from NIH/NINDS grant NS036362 and the Attilio and Olympia Ricciardi Research Fund (M.E.R). We also appreciate critical reading of the manuscript by Melissa A. Stouffer and Harry Xenias.


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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  1. 1.Departments of Neurosurgery and Physiology & NeuroscienceNew York University School of MedicineNew YorkUSA

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