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Dopamine pp 229-242 | Cite as

Regulation of Dopamine Transporter Expression by Neuronal Activity

  • Shalini Padmanabhan
  • Thach Pham
  • Balakrishna M. PrasadEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 964)

Abstract

Actions of extracellular dopamine released in the central nervous system are primarily terminated by the dopamine transporter. This protein is also a target for therapeutic and abused psychostimulant drugs. Different methods used to study dopamine transporter function, its expression, and intracellular signaling in neurons are described in this chapter. Function of the dopamine transporter in mesencephalic primary cultures can be measured by dopamine uptake assay. Expression of the transporter protein and mRNA are analyzed by western blots and quantitative RT-PCR, respectively. Finally, methods to study neuronal activity-dependent changes in Ca2+⁄calmodulin-dependent protein (CaM) kinase activity in dopamine neurons are described.

Key words

Mesencephalic Dopamine Uptake Transporter Western blot Quantitative RT-PCR Action potential Tyrosine hydroxylase CaM kinase MAP kinase 

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Copyright information

© Springer Science+Business Media, LLC 2013

Authors and Affiliations

  • Shalini Padmanabhan
    • 1
  • Thach Pham
    • 2
  • Balakrishna M. Prasad
    • 1
    • 3
    Email author
  1. 1.Department of PharmacologyMedical College of GeorgiaAugustaUSA
  2. 2.General SurgeryDwight D. Eisenhower Army Medical CenterFort GordonUSA
  3. 3.Clinical InvestigationDwight D. Eisenhower Army Medical CenterFort GordonUSA

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