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Dopamine pp 201–214Cite as

Genomic Strategies for the Identification of Dopamine Receptor Genes in Zebrafish

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 964))

Abstract

In this chapter, we describe the identification and cloning of D2-like dopamine receptor (DR) genes in zebrafish, a vertebrate model genetic organism. To identify DR genes, we performed searches of the zebrafish genomic sequence database that yielded contig segments of several D2-like DR genes. From these sequences, we amplified full-length cDNAs encoding three D2, one D3, and three D4 DR receptor subtypes via RT-PCR. The predicted proteins displayed 57–72% amino acid identity when compared to their human DR counterparts. To validate the identity of zebrafish DR genes, each of the genes was mapped by using the T51 radiation hybrid panel. With the exception of drd2b and drd4b, each of the zebrafish DR genes mapped to chromosomal positions that were syntenic with regions of human chromosomes containing orthologs of the zebrafish DR genes. To further validate the identity of the D2-like DR genes in zebrafish, we conducted phylogenetic analysis which supported the predicted identities of the cloned DR receptor cDNAs.

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Authors and Affiliations

  1. Department of Biological Sciences, York College of Pennsylvania, York, PA, USA

    Wendy Boehmler

  2. Department of Pharmacology, Penn State College of Medicine, Hershey, PA, USA

    Jessica Petko, Victor A. Canfield & Robert Levenson

Authors
  1. Wendy Boehmler
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  2. Jessica Petko
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  3. Victor A. Canfield
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  4. Robert Levenson
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Corresponding author

Correspondence to Robert Levenson .

Editor information

Editors and Affiliations

  1. Krasnow Institute for Advanced Study, Dept. of Molecular Neuroscience, George Mason University, University Drive 4400, Fairfax, 22030, Virginia, USA

    Nadine Kabbani

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Boehmler, W., Petko, J., Canfield, V.A., Levenson, R. (2013). Genomic Strategies for the Identification of Dopamine Receptor Genes in Zebrafish. In: Kabbani, N. (eds) Dopamine. Methods in Molecular Biology, vol 964. Humana Press, Totowa, NJ. https://doi.org/10.1007/978-1-62703-251-3_12

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  • DOI: https://doi.org/10.1007/978-1-62703-251-3_12

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  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-1-62703-250-6

  • Online ISBN: 978-1-62703-251-3

  • eBook Packages: Springer Protocols

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