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X-ray Structural Analysis of S100 Proteins

  • Günter Fritz
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 963)

Abstract

X-ray crystallography is a potent and meanwhile fast technique to obtain detailed structural information of S100 proteins in their apo or metal ion-loaded state. S100 proteins crystallize in the absence or presence of Ca2+ and Zn2+ and the obtained crystals often diffract to high resolution yielding information on the ion-binding sites, conformation, and target interaction sites of the proteins. Here, I describe a general scheme to isolate and crystallize S100 proteins and the analysis of protein crystals using a modern synchrotron source.

Key words

S100 protein Calcium Zinc Crystallization X-ray EF-hand 

Notes

Acknowledgments

This work was supported by grants FR14488/3-1 and FR1488/5-1 from the Deutsche Forschungsgemeinschaft (DFG).

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Department of Neuropathology, NeurozentrumUniversity of FreiburgFreiburgGermany

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