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Human Cell Line Authentication: The Critical First Step in Any Project Using Human Cell Lines

  • Robert S. McLaren
  • Yvonne ReidEmail author
  • Douglas R. Storts
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 963)

Abstract

Short tandem repeat (STR) typing is a standard procedure used in many laboratories for the authentication of human cell lines. This technology, which is based on the informativeness of known polymorphism of numerous loci to uniquely identify a human cell line, has allowed for direct-amplification of human DNA stored on FTA® paper. We describe an application of this technology to create a unique STR profile by direct amplification of HCT 116 (ATCC® CCL-247™) cell line DNA, a cell line commonly used in colon research. The ability to perform direct-amplification of DNA opens up the possibility of using FTA® paper as a way to maintain long-term storage of DNA samples from a cell line and other human tissues, such as buccal cells.

Key words

STR DNA profiling Human cell lines Authentication 

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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  • Robert S. McLaren
    • 1
  • Yvonne Reid
    • 2
    Email author
  • Douglas R. Storts
    • 1
  1. 1.Promega CorporationMadisonUSA
  2. 2.American Type Culture Collection (ATCC™)ManassasUSA

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