Culture Methods and Mutant Generation in the Filamentous Brown Algae Ectocarpus siliculosus

  • Aude Le Bail
  • Bénédicte CharrierEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 959)


Ectocarpus siliculosus is a small filamentous alga that has recently emerged as the new model for fundamental research on brown algae. Here, we describe the basic culture protocols for propagating and collecting E. siliculosus material that can then be used in all types of molecular biology, biochemistry and cell biology techniques. In addition, procedures for carrying out genetic experiments (generation of mutants and genetic segregation analyses) on E. siliculosus are described.

Key words

Brown algae Ectocarpus siliculosus Tissue culture Genetics Mutant 


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Copyright information

© Springer Science+Business Media New York 2013

Authors and Affiliations

  1. 1.Cell Biology Division, Department of BiologyUniversity of Erlangen-NurembergErlangenGermany
  2. 2.Morphogenesis of Macroalgae, UMR7139 Marine Plants and Biomolecules, CNRS-UPMCRoscoffFrance

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